Handayani, Kusuma and Santosa, Andreas and Mubarik, Rachmania and Sudadi, Untung (2019) ISOLATION AND CHARACTERIZATION OF PHOSPHATE SOLUBILIZING BACTERIA FROM PINEAPPLE RIZOSPHERE IN LAMPUNG. In: 10 International Seminar of Indonesian Society for th Microbiology (10 ISISM) th & 12 Congress Indonesian Society for Microbiology th (12 CISM), 29-30 Agustus 2019, Solo Jawa Tengah. (Unpublished)

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Abstract

Pineapple is one of the most cultivated plants that use fertilizers in its management system. Excessive use of chemical fertilizers can change soil structure and reduce soil fertility. Therefore, a method is used to address the problem, i.e. using phosphate solubilizing bacteria (PSB) found in soil around the roots (rhizosphere) of pineapple plants. PSB can dissolve bound phosphates, into exchangeable or water-soluble phosphate available for plants. The purposes of this study were to determine the number of PSB in pineapple rhizosphere, to isolate PSBwith high solubility index (SI), and to determine the characteristics of PSB isolated from pineapple rhizosphere. This study used descriptive method by taking rhizosphere soil samples from 4 different block locations at pineapple plantation in Lampung. The results showed that the PSB population was around 103 CFU/g. Out of total 12 PSB isolates tested for their P solubilizing ability, isolate RV1 had the lowest SI(0.7) while isolate RG2 had the highest (1.2). Morphological characteristics of PSB isolateswere mostly white, yellowish, gram-positive, and rod-shaped. The optimum pH for average growth was at pH 5.0, while the optimum temperature was at 30°C. Based on 16S rRNA identification, isolate RG2 was similar to Bacillus altitudinis.

Item Type:	Conference or Workshop Item (Poster)
Subjects:	Q Science > QR Microbiology
Divisions:	Fakultas Matematika dan Ilmu Pengetahuan Alam (FMIPA) > Prodi Biologi
Depositing User:	KUSUMA HAN
Date Deposited:	02 Jun 2020 13:57
Last Modified:	02 Jun 2020 13:57
URI:	http://repository.lppm.unila.ac.id/id/eprint/21500

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