Risal, Wintoko and Exsa Hadibrata, exsa and Dwita Oktaria, DO and David, Ruru (2021) The Effect of Black Cumin (Nigella Sativa) Extract to Decreasing Oxidative Stress (MDA) and Tensile Strength through on Artificial Achilles Tendon of Diabetes Mellitus Type 2 Wistar Rat. DIPA FAKULTAS KEDOKTERAN 2021. (Unpublished)

Text Risal DIPA UNILA 2021- JOURNAL.docx Download (60kB)

Abstract

Achilles tendon rupture is a condition when the Achilles tendon is injured or torn.It has been reported that the incidence of Achilles tendon rupture in patients with diabetes mellitus was 2.35 per 1000 population per year. This study aimed to determine the role of black cumin (Nigella sativa) as an antioxidant in the healing of Achilles tendon rupture in Wistar rats model with type 2 DM, with an indicator of a decrease in malondialdehyde and an increase in tensile strength of the Achilles tendon. The research design used in this study was the true laboratories experimental method with post test only control group, the samples were type 2DM and non-DM Wistar rats (Rattus norvegicus). Tenotomy and tendon repair were carried out in rats. After that, they were divided into 4 groups, namely: non-DM ratsthat were not treated with black cumin, non-DM rats treated with black cumin, DM rats that were not treated with black cumin and DM rats treated with black cumin. After three weeks, tendon sampling was carried out to measure malondialdehyde levels and tensile strength. The results showed that the highest average malondialdehyde value in the DM group that was not treated with black cumin was 493.6, and the lowest average malondialdehyde value in the non-DM group treated with black cumin was 332.0. In addition, it can be seen that the highest average tensile strength value in the non DM group treated with black cumin was 36.9, and the lowest average tensile strength value in the DM group that was not treated with black cumin was 11.8. From these data, it can be seen that the DM group treated with black cumin had lower malondialdehyde levels and higher tensile strength than the DM group who was not treated with black cumin. Based on the regression test, it was found that black cumin had a significant effect on malondialdehyde levels and tensile strength. Based on the Pearson correlation test, it was found that there was a significant relationship between malondialdehyde and tensile strength values. It can be concluded that the groups of rats that underwent artificial Achilles tendon rupture and was treated with black cumin extract had stronger tensile strength and lower malondialdehyde levels than those who were not treated with black cumin extract. In addition, there was a significant relationship between malondialdehyde levels and tensile strength. It is suggested for further studies to perform an investigation on tendon rupture in humans with type 2 DM complications, so that black cumin can be considered as adjuvant therapy for tendon rupture.

Item Type:	Other
Subjects:	R Medicine > R Medicine (General)
Divisions:	Laporan Penelitian LPPM
Depositing User:	RISAL WINT
Date Deposited:	11 Nov 2021 08:41
Last Modified:	11 Nov 2021 08:41
URI:	http://repository.lppm.unila.ac.id/id/eprint/36151

Actions (login required)

View Item