Noviany, Noviany and Ratu, Dwi Gustia Rasyidi and Andi, Setiawan (2016) Antioxidant Assay Guided Separation of the Methanol Stem Fraction of Binahong (Anredera Cordifolia) Using Cyclic Voltammetry Metho. Oriental Journal of Chemistry, 33 (1). pp. 1-8. ISSN 0970-020 X
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Abstract
The aim of this study was to identify and characterize groups of antioxidant active naturally from methanol fraction of the stem of Anredera cordifolia through antioxidant assay-guided separation. The methanol fraction (MF) displayed the highest antioxidant activity, compared to ethyl acetate fraction (EAF) and hexane fraction (HF) using cyclic voltammetry method. Ascorbic acid was used as a positive control in this research. The repeated fractionation of active MF by MPLC and HPLC analysis yielded a constituent with strong antioxidant property. The active constituent (RNV-1) was predicted as a flavonoid type based on the UV spectrum data. The antioxidant activity results exhibited that RNV-1 had a coefficient of antioxidant activity (K) sligtly higher than K of ascorbat acid with the values of 0.055 and 0.052 respectively. It was found that RNV-1 demonstrated an excellent antioxidant activity in comparison with the standard antioxidant activity through antioxidant assays-guided separation. The results obtained suggested that the stem of A. cordifolia can be used potentially as a source of natural antioxidants by contributing beneficial health effects
| Item Type: | Article |
|---|---|
| Subjects: | Q Science > Q Science (General) |
| Depositing User: | Prof Andi Setiawan |
| Date Deposited: | 03 Nov 2021 09:27 |
| Last Modified: | 03 Nov 2021 09:27 |
| URI: | http://repository.lppm.unila.ac.id/id/eprint/34928 |
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