Setyaningrum, Endah and Arifiyanto, Achmad and Nismah, Nukmal and Aeny, Titik Nur and Putri, Meishy Handerlin and Setiawati, Ulin Ni'mah In vitro Test for Inhibition of Plasmodium falciparum 3D7 Parasites using Streptomyces hygroscopicus subsp. hygroscopicus Strain i18, Isolated from a Pineapple Farm in Lampung. J Pure Appl Microbiol, 13(2), 815-821 | June 2019. ISSN 2581-690X

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Abstract

Increasing natural ingredient awareness and utilization has created increased demand for sources of natural medicinal ingredients, including sources of compound used to treat malaria. Streptomyces is a genus of prokaryote well recognized for its production of antibiotics and other pharmaceutically useful compound. This study aimed to assess the ability of unpurified fermentation metabolites to inhibit Plasmodium parasites. A strain of bacteria identified as Streptomyces hygroscopicus subsp. hygroscopicus strain i18 were isolated from pineapple fields in Lampung province, and was cultured and fermented on liquid synthetic Gause medium for 10 days. The supernatant was separated from the cells and extracted with ethyl acetate-methanol (1:1). Plasmodium falciparum 3D7 was used for antiplasmodial testing. Metabolites were tested qualitatively using a phytochemical approach. Saponins and triterpenoids were found to be present in the extract. Parasite inhibition as measured using probit analysis and yielded an IC50 value of 11.07 g.m/L. These findings suggest further examinations of this extract (e.g. assessment of off target effects) are warranted.

Item Type:	Article
Subjects:	Q Science > QR Microbiology R Medicine > R Medicine (General) R Medicine > RM Therapeutics. Pharmacology
Divisions:	Fakultas Matematika dan Ilmu Pengetahuan Alam (FMIPA) > Prodi Biologi
Depositing User:	Mr Achmad Arifiyanto
Date Deposited:	02 Jun 2021 01:08
Last Modified:	02 Jun 2021 01:08
URI:	http://repository.lppm.unila.ac.id/id/eprint/31640

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