Mahfut, Mahfut (2020) Molecular phylogeny of Tricodherma sp. Isolated from native tropical orchids in Indonesia. Biocatalys and Agricultural Biotechnology, xx (x). ISSN 1878-8181 (Submitted)

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Abstract

Tricodherma mycorrhiza has an important role as a biocontrol agent. Its association with Phalaenopsis amabilis was molecularly identified through rDNA-ITS sequence analysis. The aims of the study were to identify isolated molecular of orchids mycorrhiza from native tropical orchids in Indonesia, conducted as one of native orchid conservation efforts in Indonesia. One isolate of Tricodherma were isolated from the root of orchid plant in Yogyakarta based on morphological and microscopical analysis. Verification analysis molecular of these isolates resulted in 600-750 bp DNA products located on the ITS1-5.8S-ITS4 region. The sequenced products showed insertion and substitution occurances, which may result in strain diversity and possible variation in severity. Reconstruction of phylogenetic trees using Maximum Parsimony and Bootstrap-1000 approach showed that Indonesian isolates have undergone speciation and have been positioned in the cluster, which are already far apart from the other isolates. Isolate Tricodherma from Yogyakarta, Indonesia successfully isolated based on identification of rDNA-ITS sequences. Results of this study were expected to become the basic information in an effort of native orchid cultivation and protection against infectious diseases in Indonesia. The study was the first to report regarding Tricodherma isolated from native tropical orchids in Indonesia.

Item Type:	Article
Subjects:	Q Science > QK Botany
Divisions:	Fakultas Matematika dan Ilmu Pengetahuan Alam (FMIPA) > Prodi Biologi
Depositing User:	MAHFUT - -
Date Deposited:	29 May 2020 06:55
Last Modified:	29 May 2020 06:55
URI:	http://repository.lppm.unila.ac.id/id/eprint/21002

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