Afandi, Auliana and Ayaka, Hieno and Wibowo, Arif and Subandiyah, Siti and Afandi, . and Suga, Haruhisha and Tsuchida, Koji and Kageyama, Koji (2019) Genetic diversity of Phytophthora nicotianae reveals pathogen transmission mode in Japan. Journal of General Plant Pathology. pp. 1-12. ISSN 1610-739X

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Abstract

Phytophthora nicotianae is an important soil-borne pathogen in tropical, subtropical and temperate regions. To clarify the genetic diversity of P. nicotianae and to understand its mode of transmission in Japan, we developed six new microsatellites markers, consisting of six loci and 39 alleles. In a phylogenetic analysis, 138 isolates, including 125 from Japan and 13 from overseas, were shown to differ, even though some were collected from the same host and location, suggesting that there is no geographic or host plant clustering. Population structure analysis also revealed a highly admixed population of P. nicotianae in Japan. Molecular analysis suggested high variance between individuals but no significant differences between populations. Both A1 and A2 mating types were present in the same population, which could be due to high levels of variance between individuals in the population. The absence of geographical structure between populations also suggests that the pathogen is able to migrate from one population to another. We propose that this phenomenon could result from human activities related to the transport of plant and associated agricultural materials. Keywords Diversity · Microsatellite · Phytophthora nicotianae · Population genetics · Population structure

Item Type:	Article
Subjects:	S Agriculture > S Agriculture (General)
Divisions:	Fakultas Pertanian (FP) > Prodi Ilmu Tanah
Depositing User:	AFANDI
Date Deposited:	09 Apr 2019 06:58
Last Modified:	09 Apr 2019 06:58
URI:	http://repository.lppm.unila.ac.id/id/eprint/11106

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