Sumardi, Sumardi and Salman, Farisi and christina, Nugroho Ekowati and Cahya, Intan Listiyorini (2019) ISOLATION AND CHARACTERIZATION Bacillus sp. PRODUCING CELLULASE ENZYMES FROM HANURA MANGROVE. In: International Conference on Marine and Coastal Engineering and Sciences 2019, 23 Agustus 2019, Bandar Lampung. (Submitted)

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Abstract

In the mangrove ecosystem, there are cellulolytic bacteria that act to decompose cellulose in nature. This study aims to obtain cellulolytic bacteria from mangrove forests in Hanura village. Bacterial isolation was carried out using Sea Water Complate Agar (SWCA) media which added Carboxy Methyl Cellulose 0.5% (CMC). Characterization of isolates included colony and cell morphology, pH and NaCl stress test, pathogenicity test, and metal influence test on cellulolytic activity. The results of the study obtained 38 bacterial isolates. 16 isolates among them were cellulolytic bacteria and were able to grow well on pH (7 and 10) and NaCl (0%, 3% and 6%) stress media. IBK3, ID2K1 and IA2K3 isolates are non-pathogenic bacteria with rod-positive Gram-positive properties. IBK3 had the highest cellulolytic index of 7.36 and grew well on the addition of Fe, Al, Pb and Cu metals.

Item Type: Conference or Workshop Item (Paper)
Subjects: Q Science > QR Microbiology
Divisions: Fakultas Matematika dan Ilmu Pengetahuan Alam (FMIPA) > Prodi Magister Ilmu Biologi
Depositing User: Sumardi Sumardi
Date Deposited: 07 Nov 2019 04:38
Last Modified: 07 Nov 2019 04:38
URI: http://repository.lppm.unila.ac.id/id/eprint/15418

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