Kustyawati, Maria Erna and Astuti, Peni Puji (2018) Who produces vitamin B12 in Tempeh. In: Seminar Internasional ICGAP, 18-20 Oktober 2018, Malang. (Submitted)

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Abstract

Since 20 years ago people have known that vitamin B12 in tempeh is produced by activity of bacteria especially Klepsiella sp and Citrobacter freundii during fungal fermentation. In this study Klebsiella sp and Saccharomyces cerevisiae were used as inoculums along with Rhizopus oligosporus in soybean fermentation for the making of tempeh (T). Each inoculum was inoculated separately along with R.oligosporus on to dehulled cooked soybeans as follows: soybeans + R.oligosporus + Klebsiella sp (TRK), soybeans + R.oligosporus + S.cerevisiae (TRS), soybeans + R.oligosporus + S.cerevisiae + Klebsiella sp. (TRSK), and Soy + R.oligosporus (TR) and soybeans + Klebsiella sp. (TK). Inoculated soybeans were then incubated at 30°C for 36 hours. Observations were made on the growth of Klebsiella sp, S.cerevisiae and R. Oligosporus, and vitamin B12 in tempeh. The results showed that the highest vitamin B12 was 3.15 mg/100g in TSR, followed by 2.88 mg/100g and 1.64 mg/100g were in TSKR and TR respectively. Meanwhile, vitamin B12 in TKR, 0.81mg/100g, was lower than in TK which was 0.96mg/100g. Nevertheless, the total number of bacteria in the tempeh was the highest (108 CFU / g) compared with the total number of S. cerevisiae (104CFU / g) or the total number of R.oligosporus (103CFU / g) in the tempeh. This suggests that the role of bacteria in producing vitamin B12 was less than that of S. cerevisiae or R.oligosporus. The conclusion was that S. cerevisiae has a significant contribution to the production of vitamin B12 in tempeh.

Item Type:	Conference or Workshop Item (Paper)
Subjects:	S Agriculture > S Agriculture (General)
Divisions:	Fakultas Pertanian (FP) > Prodi Teknologi Hasil Pertanian
Depositing User:	Dr Maria Erna Kustyawati
Date Deposited:	15 Nov 2018 08:09
Last Modified:	15 Nov 2018 08:09
URI:	http://repository.lppm.unila.ac.id/id/eprint/9972

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