Wedhastri, Sri and Mindrati Fardhani1, Dinar and Kabirun, Siti and Widada, Jaka and Widianto, Donny and Evizal, Rusdi and Dwidya Prijambada, Irfan (2013) Legume Nodulating Bacterium, Achromobacter xylosoxidans Found in Tropical Shrub Agroecosystem, Sumatera, Indonesia. Maj Kedokt Indon, 18 (2).

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Abstract

Abstract Legume nodulating bacteria (LNB), known also as rhizobia, are soil bacteria, which are able to form root nodules and fi x nitrogen in the leguminous plants. The LNB availability in the soil depends on the type of agroecosystem, where plant grows. In this study, we isolated LNB from the shrub agroecosystem in Sumatera, Indonesia, and obtained four selected bacterial strains. Among them, the isolate UGM48a formed root nodule in Macroptilium atropurpureum and showed highest number of nitrogenase activity. UGM48a also contains nifH and nodA genes. An analysis of the PCR-amplifi ed 16S rDNA and BLASTn analysis showed that UGM48a displayed 96% similarity with Achromobacter xylosoxidans. In addition, UGM48a were successfully nodulated Glycine max (L.) merr var. wilis. This is the fi rst report detecting A. xylosoxidans as nodule-forming species for Glycine max possesing the positive copy of nodA gene. Keywords : Legume Nodulating Bacteria, shrub agroecosystem, Achromobacter xylosoxidans, nodA, Glycine max

Item Type:	Article
Subjects:	A General Works > AC Collections. Series. Collected works
Divisions:	Fakultas Kedokteran (FK) > Prodi Pendidikan Dokter
Depositing User:	Daud . Busrani
Date Deposited:	12 Jul 2016 01:36
Last Modified:	04 Nov 2016 03:20
URI:	http://repository.lppm.unila.ac.id/id/eprint/95

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