Rustiati, Elly L. and Priyambodo, Priyambodo and Asiyah, Siti and Candra, Dedi and Anggraini, Diah Esti and Krismuniarti, Elizabeth Devi and Srihanto, Eko Agus and Angeliya, Liza and Nurcahyani, N and Saswiyanti, Enny (2018) DNA Isolation on Captive Sumatran Elephant in Elephant Training Center, Way Kambas National Park: A First Step towards Its ID Card. Int. J. Trop. Vet. Biomed. Res., 3 (1). pp. 1-3. ISSN 2503-4715
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Abstract
Elephant Training Center (ETC) Way Kambas National Park (WKNP) was built to support human-elephant mitigation conflict. The small population of captive sumatran elephant in ETC WKNP need a comprehensive strategy in order to maintain the genetic variation of each individual and avoid inbreeding drive. Currently, genetic studies have opened new field studies in ecology, included conservation ecology. Patterns in variation of population has been investigated by molecular method supporting species conservation effort. The captive sumatran elephant’s ID Card is a necessary in database building, which included morphology, health status, and genetic profile. Genetic profile in each ID Card was filled by cytogenetic and molecular profile for RADP result, that initiated with DNA isolation. The DNA sources collected by blood sampling protocol described by Asiyah et al. (2016) from captive sumatran elephant in ETC, WKNP, and be carried to laboratory in cold condition. The DNA sources stored at 4oC and isolated following commercial protocol. The result of DNA isolation stored at -20oC until amplification analysis. DNA isolation was successfully done, for further individual genetic ID building.
| Item Type: | Article |
|---|---|
| Subjects: | Q Science > Q Science (General) Q Science > QH Natural history > QH301 Biology Q Science > QH Natural history > QH426 Genetics |
| Divisions: | Fakultas Matematika dan Ilmu Pengetahuan Alam (FMIPA) > Prodi Biologi |
| Depositing User: | Mrs Elly Lestari Rustiati |
| Date Deposited: | 26 Oct 2018 10:02 |
| Last Modified: | 26 Oct 2018 10:02 |
| URI: | http://repository.lppm.unila.ac.id/id/eprint/8872 |
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