Suhandy, Diding and Yulia, Meinilwita and Suzuki, Tetsuhito and Ogawa, Yuichi and Kondo, Naoshi (2018) RIGHT-ANGLE FLUORESCENCE SPECTROSCOPY COUPLED WITH PLS-DA FOR DISCRIMINATION OF INDONESIAN PALM CIVET COFFEE. In: ISAE International Seminar 2017, 9-12 August 2017, Bandar Lampung.
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Abstract
Indonesian palm civet coffee is regarded as one the most expensive and rarest coffee in the world. Authentication of civet coffee is recently becoming important due to high potential of adulteration and mislabelling of Indonesian palm civet coffee with cheaper non-civet coffee. In the present study, rightangle fluorescence spectroscopy coupled with partial least squares discriminant analysis (PLS-DA) were used for discrimination of Indonesian palm civet coffee. Total 60 samples were used (30 samples for civet and non-civet coffee, respectively). All coffee samples were ground using a home-coffee-grinder. Since particle size in coffee powder has a significant influence on the spectra obtained, we sieved all coffee samples through a nest of U. S. standard sieves (mesh number of 40) on a Meinzer II sieve shaker for 10 minutes to obtain a particle size of 420 μm. The experiments were carried out at room temperature (around 27-29°C). All samples were extracted with distilled water and then filtered. For each samples, 3 mL of extracted sample then was pipetted into 10 mm cuvettes for spectral data acquisition. Excitation emission spectra were obtained for civet and non-civet coffee samples by acquiring emission spectra from 210 to 750 nm (interval 1 nm) with excitation in the range of 200-550 nm (interval 5 nm) using JASCO FP-8300 Fluorescence Spectrometer. The result showed that the discrimination model was successfully developed by PLS-DA and resulted in high performance of discrimination between civet and non-civet coffee with 100% of discrimination rate. The study demonstrated that the right-angle fluorescence spectroscopy technique combined with PLS-DA method has high potential in rapid and accurate qualitative analysis of Indonesian palm civet coffee discrimination
| Item Type: | Conference or Workshop Item (Paper) |
|---|---|
| Subjects: | S Agriculture > S Agriculture (General) |
| Divisions: | Fakultas Pertanian (FP) > Prodi Teknik Pertanian |
| Depositing User: | Dr. Diding Suhandy |
| Date Deposited: | 30 Jun 2018 05:34 |
| Last Modified: | 30 Jun 2018 05:34 |
| URI: | http://repository.lppm.unila.ac.id/id/eprint/8310 |
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