Agustian, Joni and Harun@Kamaruddin, Azlina and Aboul-Eneinn, Hassa (2016) Enantio-conversion and -selectivity of racemic atenolol kinetic resolution using free Pseudomonas fluorescens lipase (Amano) conducted via transesterification reaction. RSC Advances, 6 (31). pp. 26077-26085. ISSN 2046-2069
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Abstract
In this report, effects of reaction parameters on kinetic resolution of racemic atenolol using Pseudomonas fluorescens lipase were investigated via transesterification for production of pharmacologically active eutomer (S)-atenolol with high enantiomeric purity. It was found that a temperature of 45 �C produced an acceptable enantioselectivity (E: 17). Good agitation speeds were found at 170–230 rpm producing E values of 12–15, whilst an enzyme activity of $2500 U gave 100% conversion of the (S)-atenolol. Substrate concentrations of 11.26–18.80 mM gave E values of 11.6–12.3. Variation of the substrate molar ratio yielded (S)-atenolol conversions of 44.67–61.58% with E ¼ 12–23.
| Item Type: | Article |
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| Subjects: | T Technology > TP Chemical technology |
| Divisions: | Fakultas Teknik (FT) > Prodi Teknik Kimia |
| Depositing User: | JONI AGUST |
| Date Deposited: | 04 Oct 2016 01:12 |
| Last Modified: | 04 Oct 2016 01:12 |
| URI: | http://repository.lppm.unila.ac.id/id/eprint/709 |
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