Fitriana, Yuyun and Suharjo, Radix and Rini, Maria Viva and Hidayat, Kuswanta Futas (2018) Low pH-tolerant mutant of Trichoderma spp. induced by EMS, gamma rays and UV irradiation. In: International Symposium on Innovative Crop Protection for Sustainable Agriculture 2018, 7-8 March 2018, Gifu University.

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Abstract

Basal stem rot caused by Ganoderma boninense is one of the most important problems causing severe economic losses in many oil palm industries, including in Indonesia. Recently, Trichoderma spp. has been widely used as biological control agent of G. boninense, however, this fungus was negatively affected by low pH condition. In Indonesia, oil palm commonly cultivated in marginal soil such us ultisol and peat soil with low pH (acid). In this study, three mutagens (EMS, gamma rays and UV irradiation) were used to generate low pH-tolerant mutant of Trichoderma spp. to increase its survival and effectiveness when it is applied in the previous mentioned field circumstance. Four Trichoderma isolates were used as the wild type. The mutant isolates were able to grow and produce spores in the pH 2-Potato Dextrose medium but not for the wild type. Only 1 out of 109 potentially low pH-tolerant mutant of Trichoderma showed better colony growth, sporulation, viability and antagonist to G. boninense than the wild type. These results showed that EMS, gamma rays and UV irradiation can be used to improve low-pH tolerant of Trichoderma spp.. However, the mutants must be carefully evaluated for any possibility of its negative impacts.

Item Type:	Conference or Workshop Item (Speech)
Subjects:	S Agriculture > S Agriculture (General) S Agriculture > SB Plant culture
Divisions:	Fakultas Pertanian (FP) > Prodi Proteksi Tanaman
Depositing User:	YUYUN FITRIANA
Date Deposited:	25 Apr 2018 02:24
Last Modified:	25 Apr 2018 02:24
URI:	http://repository.lppm.unila.ac.id/id/eprint/6682

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