Setiawan, Kukuh and Timotiwu, P.B. and Agustiansyah, Agustiansyah and Hadi, Muhammad Syamsoel and Ardian, . and Setiawan, A. Wawan (2017) Characterization of cassava starch and detection of starch synthase gene under different micro nutrient fertilizer levels by using scanning electronic microscopy (SEM) and Real Time PCR. In: ISAE International Seminar 2017, 9-12 August 2017, Bandar Lampung. (In Press)

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Abstract

The objectives of this study were to evaluate vegetative growth and root storage of cassava applied by micro nutrient fertilizer, to compare root storage of yield treated by micro nutrient fertilizer as 0, 20, and 40 kg/ha, to evaluate the structure of cassava starch by using Scanning Electron Microscopy (SEM), and to identify the activity of gene starch synthase with Real Time PCR. There were three different dosages of micro nutrient fertilizer as 0, 20, and 40 kg/ha. Variables observe in this study were leaf number, leaf dry weight, storage root number, storage root wet weight, diameter of starch granule, visual starch granule by SEM, and gene starch synthase activity by Real Time PCR. The result showed that leaf number of cassava applied by high dosages of micro nutrient fertilizer (20 & 40 kg/ha) tended to be lower compared to those without micro nutrient fertilizer application. Yet, the application of high dosages of micro nutrient fertilizer, tended to produce relatively high leaf dry weight. Such condition could support the production of high storage root number and weight. Additionally, starch structure applied by high dosages of micro nutrient fertilizer seemed to be denser due probably to high starch granule diameter. The increase in storage root number and density of cassava applied by 20 kg micro nutrient fertilizer/ha was concomitant with high gene starch synthase activity, approximately as many as 200 times compared to those without micro nutrient fertilizer application. Keywords: micro nutrient fertilizer, Real Time PCR, starch granule, starch synthase

Item Type:	Conference or Workshop Item (Paper)
Subjects:	S Agriculture > S Agriculture (General)
Divisions:	Fakultas Pertanian (FP) > Prodi Agronomi dan Hortikultura
Depositing User:	Kukuh Setiawan
Date Deposited:	16 Nov 2017 07:00
Last Modified:	16 Nov 2017 07:00
URI:	http://repository.lppm.unila.ac.id/id/eprint/5697

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