Rafian, Teguh and Yurnalis, Yurnalis and Fenita, Yosi and Iskandarsyah, Raffi (2022) Polymorphism of Prolactin Gene (PRL/PstI) In Sikumbang Jonti Duck Using PCR-RFLP Methods. Jurnal Sain Peternakan Indonesia, 17 (3). pp. 170-174. ISSN 2528-7109
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Abstract
This study is aimed to determine polymorphism of the Prolactin gene (PRL|PstI) in Sikumbang Jonti ducks using PCR-RFLP (Polymerase Chain Reaction–Restriction Fragment Length Polymorphism) method. This study used 56 Sikumbang Jonti ducks whose blood samples were taken. Gene amplification used a pair of primers forward 5' TGC AAA CCA TAA AAG AAA AGA 3' and reverse 5' CAA TGA AAA GTG GCA AAG CAA 3', which resulted in a 400 bp fragment in exon 5 of the Prolactin (PRL) gene. The amplification product was restricted using the PstI enzyme, which recognizes the truncation site (5' G↓ACGTC 3'). From 56 samples of Sikumbang Jonti ducks identified, just one genotype was found, homozygous (-/-) with only one allele (-). Analysis of the restriction product in Sikumbang Jonti ducks obtained a uniform genetic variation of PRL|PstI (monomorphic) with an allele frequency (-) of 100%.
| Item Type: | Article |
|---|---|
| Subjects: | S Agriculture > SF Animal culture |
| Divisions: | Fakultas Pertanian (FP) > Prodi Peternakan |
| Depositing User: | Unnamed user with email teguh.rafian@fp.unila.ac.id |
| Date Deposited: | 17 Feb 2024 03:36 |
| Last Modified: | 17 Feb 2024 03:36 |
| URI: | http://repository.lppm.unila.ac.id/id/eprint/53198 |
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