Dakhlan, A. and Putra, WPB and Santosa, Purnama Edy and Kurniawati, Dian (2023) Novel Polymorphisms in Caprine Myostatin Gene and its Relationship with Growth Traits in Saburai Does (Capra hircus). Pakistan Journal of Biological Sciences, 26 (7). pp. 380-385. ISSN 10288880

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Abstract

Background and Objective: Saburai goat (Capra hircus) is a crossbred goat from Boer buck (75%) and Ettawa doe (25%) for meat production purposes. This study was carried out to detect the mutation points or Single Nucleotide Polymorphisms (SNPs) in the myostatin (MSTN) gene of Saburai with the forward sequencing method. Materials and Methods: A total of twenty-one blood samples of Saburai does (75% Boer, 25% Ettawa) were collected from the Villager Breeder Center (VBC) at Tanggamus Regency of Lampung Province, Indonesia. The DNA analysis consists of DNA isolation, PCR analysis and sequencing analysis. The record data were used for association study with the mathematical model: Yij = μ+Gi+Єij. Results: Research showed that one common SNP of g.217_218.indel.TTTTA (5'UTR) and three novel SNPs of c.386G>C (exon 1), g.641_642.indel.T (intron 2) and c.4957G>C (exon 3) were detected in the present study. In this study, a novel SNP on exon 1 and intron 2 of Saburai MSTN gene has a moderate PIC value (>0.30). In addition, a novel SNP on exon 1 and exon 3 of the Saburai MSTN gene was detected as a missense mutation of A55P and A43P, respectively. Goats with the heterozygous genotype have higher growth traits compared to goats with the homozygous genotype. Conclusion: The goats with heterozygous genotypes can be further developed to increase the productivity of Saburai goats.

Item Type:	Article
Subjects:	S Agriculture > SF Animal culture
Divisions:	Fakultas Pertanian (FP) > Prodi Peternakan
Depositing User:	Dr. Akhmad Dakhlan
Date Deposited:	28 Oct 2023 05:52
Last Modified:	28 Oct 2023 05:52
URI:	http://repository.lppm.unila.ac.id/id/eprint/52706

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