Rahmawati, Fauzia and Nurcahyani, Endang and Tundjung, Tripeni Handayani and Wahyuningsih, Sri (2023) Response of Atonic Solution on Growth of Pea (Pisum sativum L.) Plantlets under Drought Stress Conditions using PEG 6000 in vitro. Indonesian Journal of Biotechnology and Biodiversity, 7 (2). pp. 51-58. ISSN 2581-0014
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3. FAUZIA RAHMAWATI-ENDANG NURCAHYANI-IJOBB-OKTOBER 2023.pdf Download (337kB) | Preview |
Abstract
Peas (Pisum sativum L.) are plants that grow in the highlands which can be consumed as vegetables because they contain high levels of nutrients and protein. In 2018 Indonesia experienced a decrease in the number of pea exports due to decreased supply and continuity caused by environmental factors such as pests and diseases and water shortages. The purposes of this study was to determine the optimum concentration of atonic solution for selecting pea planlets, the concentration of PEG that was tolerant to selecting pea planlets, and the interaction of atonic solution and PEG 6000 that was tolerant to growth and chlorophyll content of pea planlet in vitro. This study was arranged using the basic pattern of a 3x3 factorial design with two factors, namely factor A : Atonic with three concentration levels, namely 0 ml/l (A1), 1 ml/l (A2), 2 ml/l (A3) and factor B : PEG 6000 b/v with three concentration levels, namely 0% (B1), 70% (B2), 80% (B3). Each concentration was repeated 4 times and each replicate consisted of 2 planlets of pea seeds in each culture bottle. The results showed the optimum concentration of atonic solution for selecting pea planlets against drought stress was 2 ml/l, while the tolerant concentration of PEG 6000 for selecting pea planlets was 70%, there was also an interaction between atonic 2 ml/l and PEG 6000 70% in increasing growth and chlorophyll content of pea planlets in vitro. Keywords: Pisum sativum L., Atonic, Murashige and Skoog, Stress, Poliethylen glicol.
| Item Type: | Article |
|---|---|
| Subjects: | Q Science > Q Science (General) |
| Divisions: | Fakultas Matematika dan Ilmu Pengetahuan Alam (FMIPA) > Prodi Biologi |
| Depositing User: | ENDANG NURCAHYANI |
| Date Deposited: | 09 Oct 2023 01:15 |
| Last Modified: | 09 Oct 2023 01:15 |
| URI: | http://repository.lppm.unila.ac.id/id/eprint/52583 |
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