Sutyarso, Sutyarso and Damayanti, Ayu Fikri and Busman, Hendri and Kanedi, Mohammad (2023) Effect of Squid Ink Extract on Testis Histological Parameters in D-Galactose-Induced Aging Mice. International Journal of Pharmaceutical Research and Applications, 8 (5). pp. 382-386. ISSN 2249-7781

Preview

Text Effect of Squid Ink Extract on Testis Histological Parameters in D Galactose Induced Aging Mice.pdf Download (137kB) | Preview

Abstract

Squid ink is among the marinederived substance that is known to have many bioactive properties such as antioxidant, anticancer activity, antimicrobial activity, and anti-inflammatory activity. Through its antioxidant properties squid ink was suggested to have therapeutic potential for reproductive damages. This study is an attempt to evaluate whether squid ink has therapeutic benefits against age-related decreases in testicular histology parameters in D-galactoseinduced aging male mice. Four groups of male mice were treated as follows. Group-1 received only standar food but no D-galactose induction nor squid ink given. Group-2 received D-galactose induction but not squid ink. Group-3 and group-4 received Dgalactose induction and squid ink at the dose of 40 and 100 ml/kg body weight respectively. After 35 days of treatment all test mice were sacrificed. To assess testicular histology parameters left testis from each mouse was fixed in Bouin’s solution. Transverse sections of the organs were cut at 5 μm and stained with hematoxylin and eosin and examined under a light microscope. The results, squid ink extract at a dose of 100 ml/kg body weight significantly increase testis histological parameter values in the D-galactose-induced aging mouse model. However, the recovery rate of the seminiferous tubule epithelium thickness, number of spermatogonia, primary spermatocytes and spermatids is still far below the values of normal mice. Thus, it can be concluded that squid ink has the potential to prevent age-related testis histological damages in mice but is less effective in restoring them.

Item Type:	Article
Subjects:	Q Science > QL Zoology
Divisions:	Fakultas Matematika dan Ilmu Pengetahuan Alam (FMIPA) > Prodi Biologi
Depositing User:	SUTYARSO
Date Deposited:	27 Sep 2023 08:59
Last Modified:	27 Sep 2023 08:59
URI:	http://repository.lppm.unila.ac.id/id/eprint/52509

Actions (login required)

View Item