Setyawan, Agus and Juliasih, Ni Luh Gede Ratna and Darmawan, M. and Susanto, Gregorius Nugroho and Sarida, Munti (2023) Screening, characterization, and identification of fucoidanase producing bacteria from Sargassum polycystum. AACL Bioflux, 16 (3). pp. 1357-1371. ISSN 1844-9166
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Abstract
Fucoidanase, an enzyme that catalyzes the cleavage of glycoside bonds between sulfated fucose residues in fucoidan, is found in various marine organisms, including bacteria, fungi, and invertebrates. However, there are few studies on the use of the symbiont Sargassum polycystum as a fucoidanase producer. This study aims to isolate, characterize, and identify fucoidanase-producing bacteria from S. polycystum morphologically, biochemically, and molecularly using 16S rDNA polymerase chain reaction (PCR). Isolation of bacterial symbionts was carried out by 2 methods, namely the spread plate and streak plate methods. The pure isolate was tested qualitatively and semi-quantitatively on media selection to see the clear zone produced after the administration of 10% cetyl pyridinium chloride (CPC) solution. Subsequent activity tests were conducted quantitatively, to determine the specific fucoidanase activity produced. The results showed that 4 isolates of symbiotic bacteria from S. polycystum had the greatest fucoidanase activity tested by the CPC method. The following isolates had the greatest fucoidanase activity index values: GSD (10.66±2.26), PTF (8.69±0.36), PSA (8.23±2.64), and GSB (7.92±0.11). The GSD isolate had the greatest activity of fucoidanase production at 48 and 72 h compared to the other 3 isolates, namely 0.0072 U mg-1 and 0.0083 U mg-1, respectively. Morphological, biochemical, and molecular identification with 16S rDNA PCR found that the three isolates with the greatest fucoidanase activity were Cytobacillus kochii (GSD), Bacillus cereus (PTF), and Brevibacterium sediminis (PSA) bacteria with 99% similarity. The fucoidanase produced is expected to be used for industrial-scale enzyme production in Indonesia. In addition, the use of symbiotic bacteria in brown algae is expected to reduce the exploitation of S. polycystum and contribute to the preservation of marine ecosystems.
| Item Type: | Article |
|---|---|
| Subjects: | S Agriculture > SH Aquaculture. Fisheries. Angling |
| Depositing User: | AGUS SETYA |
| Date Deposited: | 19 Jun 2023 01:17 |
| Last Modified: | 19 Jun 2023 01:17 |
| URI: | http://repository.lppm.unila.ac.id/id/eprint/52165 |
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