Setiawan, Andi and Widyastuti, Widyastuti and Irawan, Arik and Wijaya, Oklis Syahrin and Laila, Aspita and Setiawan, Wawan Abdullah and Juliasih, Ni Luh Gede Ratna and Nonaka, Kenichi and Arai, Masayoshi and Hendri, John Solid State Fermentation of Shrimp Shell Waste Using Pseudonocardia carboxydivorans 18A13O1 to Produce Bioactive Metabolites. Fermentation. ISSN 23115637

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Abstract

Marine actinomycetes are prolific microorganisms; however, knowledge of their diversity, distribution, and secondary metabolites is limited. Marine actinomycetes represent an untapped source of novel bioactive compounds. In this study, we investigated shrimp shell as substrates for model production bioactive metabolites from actinomycetes under solid state fermentation (SSF) conditions. A total of fifteen actinomycetes were isolated from six sponges and one tunicate. The isolated actinomycetes were grown on solid shrimp shells. Cultures of actinomycetes were extracted with ethyl acetate (EtOAc) and extracts were bioassayed for activity against Staphylococcus aureus. One isolate 18A13O1 from the sponge, Rhabdastrella globostellata, exhibited antibacterial activity on primary screening compared to the other samples and was chosen for further study. Visualization using SEM showed aerial and substrate mycelia. Through phylogenetic analysis, it was confirmed that isolate 18A13O1 is a Pseudonocardia carboxydivorans. Purification of an EtOAc extract yielded A13B2, which showed a minimum inhibition concentration against S. aureus at 15.6 µg/mL. It can be concluded that this basic information is very important for further studies related to the development of the production of bioactive secondary metabolites through the solid state fermentation process. Keywords: actinomycetes; antibacteria; Staphylococcus aureus; solid state cultivation

Item Type:	Article
Subjects:	Q Science > Q Science (General)
Divisions:	Fakultas Matematika dan Ilmu Pengetahuan Alam (FMIPA) > Prodi Kimia
Depositing User:	ASPITA LAILA
Date Deposited:	13 Apr 2023 03:41
Last Modified:	13 Apr 2023 03:41
URI:	http://repository.lppm.unila.ac.id/id/eprint/50459

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