Yelli, Fitri and SETYO DWI, UTOMO and agustin, titin and pathak, ashutosh (2023) Somatic embryogenesis in two cassava (Manihot esculenta Crantz)genotypes. Notulae Botanicae Horti Agrobotanici Clucj-Napoca, 51 (1). pp. 1-13. ISSN 1842-4309
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Abstract
Plant breeding through hybridization in cassava is facing a problem due to inconsistent flowering, and also the donor genes controlling superior traits are limited. An alternative method of breeding is through genetic transformation, and regeneration via somatic embryogenesis is a promising route to achieve this. As somatic embryogenesis in cassava is genotype-specific, in the present study a protocol has been developed for UJ-3 and BW-1 genotypes. Immature sterile leaves from 7-10 days axillary shoots in a pre-condition medium were used as an explant. Leaves were inoculated on Murashige and Skoog (MS) medium containing picloram(0.0, 7.5, 10.0, 12.5, and 15.0 mg/L) and 1-naphthalene acetic acid (NAA, 6 mg/L) for induction of somatic embryos (SEs). Genotype BW-1 showed the best results as early callus formation time i.e. 8.04±0.32 days after induction (dai) compared to UJ-3 (8.67±2.13 dai). The callus fresh weight (0.64 g) was also higher in BW-1 than UJ-3 (0.38 g) after 4 weeks in the callus induction medium (CIM), and the callus formation ranges between 85.19±3.70 to 96.30±3.70% for both genotypes. Subculturing embryogenic callus to MS+CuSO4 (4 μM) + picloram (6 mg/L) +NAA (0.5 mg/L) (SK1 medium) germinated maximum SEs in BW-1 (46.56±36.86), whereas the number was less for UJ-3 (11.89±11.90). Further, shoots were developed from green cotyledons followed by hardening and acclimatization of plantlets
| Item Type: | Article |
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| Subjects: | S Agriculture > SB Plant culture |
| Divisions: | Fakultas Pertanian (FP) > Prodi Agroteknolgi |
| Depositing User: | Ms fitri yelli |
| Date Deposited: | 06 Mar 2023 01:55 |
| Last Modified: | 06 Mar 2023 01:55 |
| URI: | http://repository.lppm.unila.ac.id/id/eprint/48710 |
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