Setiawan, Andi and Lutfiah, Rosyidatul and Juliasih, Ni Luh Gede Ratna and Setiawan, Wawan Abdullah and Hendri, John and Arai, Masayoshi (2022) Antibacterial activity of EtOAc extract from marine-derived fungus Aspergillus nomiae A12-RF against clinical pathogen bacteria, Staphylococcus aureus. AACL Bioflux, 15 (3). pp. 1413-1421. ISSN 1844-9166
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Abstract
Sponge-derived fungi are a potential source for obtaining bioactive secondary metabolites. The aim of the study was to evaluate the in vitro antibacterial activity extract from sponge-derived fungi that could inhibit clinical pathogenic bacteria. In this study, nine isolated fungi were selected from deposite of Integrated Laboratory of Innovation and Technology Center, Lampung University. All isolates were maintained in malt extract media. Based on phylogenetic sequencing results, isolate 18A12RF was Aspergillus nomius (603 bp) using ITS1-5.8-ITS2. The isolate A12RF was cultivated and co-cultivated on rice solid media in 4 L Erlenmeyer flask to obtain 4.2 g of ethyl acetate extract (EtOAc). After that, the extract was subjected to several chromatographic steps based on bioassay-guided separation. The results of the fractionation of 2.4 g of EtOAc extract obtained 33.8 mg of the active fraction of A12RFBF3. The minimum inhibition concentration (MIC) test for the A12RFBF3 fraction showed inhibition of the growth of clinical bacteria Staphylococcus aureus at a concentration of 6.25 µg mL-1 . The findings of this study concluded that the crude extract prepared from the A12RF has antibacterial properties against clinical bacteria. This study is an important work as initial information for further studies in the search for new bioactive compounds. Key Words: antibacterial agent, Aspergillus nomiae, bacterial pathogen, marine fungi.
| Item Type: | Article |
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| Subjects: | Q Science > Q Science (General) |
| Divisions: | Fakultas Matematika dan Ilmu Pengetahuan Alam (FMIPA) > Prodi Kimia |
| Depositing User: | JOHN HENDRI |
| Date Deposited: | 29 Oct 2022 11:27 |
| Last Modified: | 29 Oct 2022 11:27 |
| URI: | http://repository.lppm.unila.ac.id/id/eprint/46061 |
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