Yandri, Yandri and Ropingi, Hendri and Suhartati, Tati and Hendri, John and Irawan, Bambang and Hadi, Sutopo (2022) The Effect of Zeolite/Chitosan Hybrid Matrix for Thermal-stabilization Enhancement on the Immobilization of Aspergillus fumigatus α-Amylase. Emerging Science Journal, 6 (3).
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12. The Effect of Zeolite_Chitosan Hybrid Matrix for Thermal-stabilization __Enhancement on the Immobilization of Aspergillus fumigatus α-Amylase .pdf Download (1MB) | Preview |
Abstract
In this paper, the A. fumigatus α-amylase had been immobilized onto zeolite/chitosan hybrid to improve its thermal-stabilization for industrial needs. The methods applied enzyme production, isolation, partial purification, immobilization, and characterization. The optimum temperatures of the native and immobilized enzymes were 50 and 55 ̊C, respectively. The native enzyme has KM of 3.478 ± 0.271 mg mL-1 substrate and Vmax of 2.211± 0.096 μmole mL-1 min-1, while the immobilized enzyme has KM value of 12.051 ± 4.949 mg mL-1 substrate and Vmax of 1.602 ± 0.576 μmole mL-1 min-1. The residual activity of the immobilized enzyme retained up 10.97% after fifth reuse cycles.The native enzyme has ΔGi of 104.35 ± 1.09 kJ mole-1 and t1⁄2 of 38.75 ± 1.53 min, while the immobilized enzyme has ΔGi of 108.03 ± 0.05 kJ mole-1 and t1⁄2 of 180.03 ± 3.31 min. According to the increase in half-life (t1⁄2), stability improvement of the A. fumigatus α-amylase was 4.65 times greater than the native enzyme. Thus, the zeolite/chitosan hybrid is used as a new supporting matrix for further enzyme immobilization to stabilize the enzymes.
| Item Type: | Article |
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| Subjects: | Q Science > QD Chemistry |
| Divisions: | Fakultas Matematika dan Ilmu Pengetahuan Alam (FMIPA) > Prodi Biologi |
| Depositing User: | BAMBANG IR |
| Date Deposited: | 06 Oct 2022 05:22 |
| Last Modified: | 06 Oct 2022 05:22 |
| URI: | http://repository.lppm.unila.ac.id/id/eprint/45538 |
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