Nurcahyani, Endang and Agustrina, Rochmah and Handayani, T.T. (2016) In Vitro Selection on Fusaric Acid of Spathoglottis plicata Bl Plantlets for Obtaining a Resistant Cultivar toward to Fusarium oxysporum. BKS-PTN Wilayah Barat Semirata 2016 Bidang MIPA Semirata 2016 Bidang MIPA Semirata 2016 Bidang MIPA. ISSN 978-602-71798-1-3
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In Vitro Selection on Fusaric Acid of Sphatoglotis plicatat BI Planlet.pdf Download (1MB) | Preview |
Abstract
The most production constraints on Spathoglottis plicata Bl. cultivation one of them caused by Fusarium wilt disease. This disease is caused by Fusarium oxysporum (Fo) and until now still can not be effectively addressed. The use of Fo resistant cultivars orchid soil with high yield expected is an important alternative disease control. A resistant S. plicata plantlet to Fo has been initiated by in vitro selection on Vacin & Went (VW) medium containing fusaric acid (FA) on selective concentration. The goals of research were to study and determine of: The FA concentration of plantlet selection tolerant to steady growth; The selection produces S. plicata were insensitive to the FA. In vitro regenerated S. plicata plantlet has done by using shoot tip explants, cultivated on the VW medium containing BAP on the concentration is 1,0 mg/L. Plantlet was selected with FA supplementation to VW solid medium in different concentration (0, 10, 20, 30 and 40 ppm) for 4 weeks. The resistance was evaluated to plantlet of one month old after which was inoculated by Fo. Results showed that: The FA tolerant concentration for plantlet selection with S. plicata steady growth is between 10-40 ppm; The selection produces plantlets S. plicata amount equal to 100% (10-30 ppm) and 86.7% (40 ppm) were insensitive to the FA.
| Item Type: | Article |
|---|---|
| Subjects: | Q Science > QK Botany |
| Divisions: | Fakultas Matematika dan Ilmu Pengetahuan Alam (FMIPA) > Prodi Biologi |
| Depositing User: | ENDANG NURCAHYANI |
| Date Deposited: | 20 Jul 2017 09:07 |
| Last Modified: | 20 Jul 2017 09:07 |
| URI: | http://repository.lppm.unila.ac.id/id/eprint/3680 |
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