Mahfut, Mahfut and Daryono, Budi Setiadi and Indrianto, Ari and Somowiyarjo, Susamto (2019) Plant-Virus Interaction on Orchids Infected Odontoglossum ringspot virus (ORSV) in Bogor Botanical Garden, Indonesia. In: 1st International Conference on Science and Technology, ICOST 2019, 2-3 May 2019, Makassar, Indonesia.
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Abstract
Biotic stress by Odontoglossum ringspot virus (ORSV) infection causes a decrease in the rate of photosynthesis. This study aimed to determine the pattern of plant-virus interactions through analysis of rbcL and coat protein genes. Virus source was collected from Bogor Botanical Garden. Detection of chloroplast and ORSV using Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR) and Polymerase Chain Reaction (PCR), respectively. Analyzed change of nucleotide and amino acid sequences using DNASTAR and MEGA Beta programs. Chloroplast DNA and virus RNA amplification managed to get amplicons at ±599 bp and ±474 bp, respectively. Sequence nucleotides analysis showed point mutations with increased synthesis of Ser, Glu, Pro, dan Arg amino acids. This study revealed a change in sequence of nucleotides and amino acids were thought to be adaptations and physiological responses of host-virus to natural environments in Indonesia.
| Item Type: | Conference or Workshop Item (Paper) |
|---|---|
| Subjects: | Q Science > QK Botany |
| Divisions: | Fakultas Matematika dan Ilmu Pengetahuan Alam (FMIPA) > Prodi Biologi |
| Depositing User: | MAHFUT - - |
| Date Deposited: | 04 Jun 2021 01:36 |
| Last Modified: | 04 Jun 2021 01:36 |
| URI: | http://repository.lppm.unila.ac.id/id/eprint/31968 |
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