Permata, Fajar Shodiq and Susilowati, Rina and Dharmastiti, Rini and Sirat, Muhammad Mirandy Pratama and Kartikasari, Pipin Dwi (2012) Optimasi Metode Deselularisasi Jaringan Berdasarkan Pengamatan Makroskopik dan Mikroskopik Saraf Tepi Domba. Jurnal Sain Veteriner, 30 (2). pp. 61-70. ISSN 0126-0421

Text Jurnal Sain Veteriner 30(2) Desember 2012 (Muhammad Mirandy Pratama Sirat).pdf - Published Version Restricted to Registered users only Available under License Creative Commons Attribution Share Alike. Download (13MB) | Request a copy

Abstract

Animal graft tissue (xenograft) was developed to replace the limited supply of human graft tissue (allograft). Peripheral nerve graft tissue is needed to replace the damage. Swine is the most developed source of tissue donor for the preparation of acellular tissue because a lot of livestock population and its extracellular matrix components similar to human collagen. Swine xenograft development would be an obstacle in Indonesia because of socio-cultural so that sheep used as swine donor replacement. Sheep cartilage tissue acellular induced less human inflammatory mediators than swine. Xenograft is necessary to decellularize before implantation into human that results in only extracellular matrix. However, decelluarization process varies depending on the species and methods so that it is needed preliminary study to get the best decellularization method for sheep peripheral nerve specimens. Five samples of sheep Ischiadicus nerves were conducted decellularization various processes and one sample of fresh nerve (control). Decellularization methods were 24 hours shaking, tissue soaking and perfusion for both 14 days and 17 days, respectively. Decellularization solution was 0.1% SDS-EDTA in PBS. Post decellularization, samples were observed macroscopically, fixed, HE staining of histopathologic examination for microscopic examination. The data were analyzed descriptively. At the macroscopic observation of post decellularization tissue showed white, and samples of soaking method showed softer consistency than that of shaking and perfusion. Microscopic examination showed that samples were not being completed decellularization at shaking method. There was destruction of collagen fibers of perinerium and endoneurium in 17 days of soaking and perfusion methods, while 14 days soaking and perfusion methods showed that samples were being sompleted decellularization and both perineurium and endoneurium collagen were still good. Conclusion of this study is that 14 days soaking and perfusion decellularization methods are better than that 24 hours shaking and 17 days of soaking and perfusion methods for sheep decellularization peripheral nerve.

Item Type:	Article
Uncontrolled Keywords:	Keywords : peripheral nerve, sheep, decellularization, macroscopic, microscopic
Subjects:	S Agriculture > SF Animal culture
Divisions:	Fakultas Pertanian (FP) > Prodi Nutrisi dan Teknologi Pakan Ternak
Depositing User:	drh.,M.Sc. Muhammad Mirandy Pratama Sirat
Date Deposited:	28 Apr 2021 01:04
Last Modified:	28 Apr 2021 01:04
URI:	http://repository.lppm.unila.ac.id/id/eprint/29643

Actions (login required)

View Item