Suharjo, Radix and Sawada, Hiroyuki and Takikawa, Yuichi (2014) Unusual Pectobacterium wasabiae strains isolated from potato in Japan. Japanese Journal of Phytopathology, 80 (4). p. 327. ISSN 0031-9473

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Abstract

During the revision of Japanese Pectobacterium strains, we have demonstrated that some strains of Erwinia carotovora from potato were identified as P. wasabiae. They had different phenotypic properties from P. wasabiae strains isolated from wasabi. Through phylogenetic analysis using recA, mdh, rpoD and gyrB, the potato strains formed slightly independent cluster from the wasabi strains. The BOX and ERIC PCR clearly showed the difference between these two groups. On wasabi rhizomes, the potato strains showed less virulent reaction than the wasabi strains, and vice versa on potato. Those two groups can be discriminated using newly designed specific primers.The primers produced amplicons of 252bp for potato strains and 427bp for wasabi strains. Thus, we concluded that the potato strains were different from the wasabi strains and may constitutes a new subspecies-level taxon in P. wasabiae.

Item Type: Article
Subjects: S Agriculture > S Agriculture (General)
Divisions: Fakultas Pertanian (FP) > Prodi Proteksi Tanaman
Depositing User: RADIX SUHA
Date Deposited: 02 Jun 2017 12:15
Last Modified: 02 Jun 2017 12:15
URI: http://repository.lppm.unila.ac.id/id/eprint/2812

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