Yandri, Yandri and Suhartati, Tati and Satria, Heri and Hadi, Sutopo (2021) Production, purification, and characterization of the α-amylase from local bacteria isolate Bacillus subtilis ITBCCB148. Journal of Physics: Conference Series, 1751 (012096). ISSN Print : 1742-6588 Online : 1742-6596
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Abstract
Production, purification and characterization of the α-amylase from local bacteria isolate Bacillus subtilis ITBCCB148 has been successfully carried out. The purified enzyme increased 149.9 times with specific activity of 115,500 U/mg compared to enzyme crude extracts. This enzyme has an optimum pH of 5.5 and an optimum temperature of 60oC and can maintain its stability at a temperature of 60-80oC, thus this enzyme is categorized as a thermostable enzyme.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | α-amylase, B. subtilis ITBCCB148, characterization, purification |
| Subjects: | Q Science > QD Chemistry |
| Divisions: | Fakultas Matematika dan Ilmu Pengetahuan Alam (FMIPA) > Prodi Kimia |
| Depositing User: | Prof. Sutopo Hadi |
| Date Deposited: | 01 Feb 2021 04:53 |
| Last Modified: | 01 Feb 2021 04:53 |
| URI: | http://repository.lppm.unila.ac.id/id/eprint/27568 |
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