Suharjo, Radix and Sawada, Hiroyuki and Takikawa, Yuichi (2013) A new rapid identification method for Japanese Pectobacterium strains based on recA, mdh and rpoD PCR RFLP. In: 10th international conggress of plant pathology, August 25 - 30, Beijing, China.
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Abstract
Identification was performed on 189 Japanese Pectobacterium strains of MAFF collection isolated from Crucifers and Solanaceous plants using sequence analysis of recA, mdh, gyrB, rpoD and PCR RFLP of recA, mdh and rpoD. Using recA, mdh and rpoD PCR RFLP each species and subspecies group of Japanese Pectobacterium strains can be easily distinguished. The results of recA, mdh and rpoD PCR RFLP was easy to analyze and corresponded to the result of recA, mdh, gyrB and rpoD sequence analysis. Using those methods, the investigated Japanese Pectobacterium strains were divided into P. carotovorum subsp. carotovorum, subsp. odoriferum, subsp. brasiliense, new subsp. level group of P. carotovorum, P. atrosepticum and P. wasabiae. Here we also found a group that may constitute a new species level group of Pectobacterium. This recA, mdh and rpoD PCR RFLP can be potentially used as rapid method for identification of Pectobacterium strains
| Item Type: | Conference or Workshop Item (Poster) |
|---|---|
| Subjects: | S Agriculture > S Agriculture (General) |
| Divisions: | Fakultas Pertanian (FP) > Prodi Proteksi Tanaman |
| Depositing User: | RADIX SUHA |
| Date Deposited: | 02 Jun 2017 12:04 |
| Last Modified: | 02 Jun 2017 12:04 |
| URI: | http://repository.lppm.unila.ac.id/id/eprint/2753 |
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