Kurniati, Intanri and Tjiptaningrum, Agustyas and Hidayat, Hidayat (2020) CORRELATION BETWEEN NEUTROPHIL LYMPHOCYTE RATIO AND CYCLE THRESHOLD VALUE OF RT PCR IN PATIENTS CONFIRMED COVID-19 IN LAMPUNG. [Experiment] (Submitted)

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Abstract

Purpose: The purpose of this study was to see a correlation between the Neutrophil Lymphocyte Ratio (NLR) and the Cycle Threshold (Ct) value of the Reverse-Transcriptase Polymerase Chain Reaction in patients who were confirmed positive for COVID-19 in laboratory of Dr. H Abdul Moeleok Hospital in Lampung Province. Research Methodology: This research is an analytical study of the cross-sectional method using secondary data where all data were taken from July-September in the hematology and biomolecular laboratory of Dr. H Abdul Moeloek Hospital. The total number data for patients who have NLR is 59. The NLR value is obtained by dividing the absolute neutrophil count by the absolute lymphocyte count using Mindray's BC 6200 automatic hematology tool. Primers used by the PCR tool Abbot M2000sp detected 2 of 3 genes (ORF 1a, N, and E). A Ct value is considered positive for COVID-19 if it is less than the threshold of 40. The number of samples that had NLR and Ct gene ORF 1a was 38. The number of samples that had NLR and Ct gene N values was 56. The number of samples that had NLR and Ct gene E was 28. The correlation test between the NLR and the Ct value used the Spearman correlation test. Results: The results of the Spearman correlation test between the NLR and the Ct value of the ORF 1a gene were (r) = -0.231 with a significance value () of 0.163. The results of the Spearman correlation test between NLR and the Ct value of the N gene were -0.173 with a significance value of () = 0.201. The result of the Spearman correlation test between the NLR and the Ct gene E value was -0.023 with a significance value of () = 0.908. These results can be concluded that there is a weak and insignificant negative correlation between NLR and the Ct value of the three specific SARSCoV-2 genes that cause COVID-19. Limitations: The limitations of this study are that it does not analyze comorbid risk factors that can affect the patient's NLR other than SarsCoV-2 infection and do not analyze the swab sampling method for RT PCR. Contribution: The results of this study are expected to provide an initial contribution to further research in the form of hematological examination results, namely NLR, which is relatively simpler as a prediction of monitoring the course of COVID-19. Keywords: Confirmed COVID-19, Cycling Threshold value, Neutrophil Lymphocyte Ratio

Item Type:	Experiment
Subjects:	R Medicine > RB Pathology
Divisions:	Fakultas Kedokteran (FK) > Prodi Pendidikan Dokter
Depositing User:	INTANRI KU
Date Deposited:	23 Nov 2020 07:58
Last Modified:	23 Nov 2020 07:58
URI:	http://repository.lppm.unila.ac.id/id/eprint/26662

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