Pritania, Savira Ananda and Rustiati, Elly L. and Priyambodo, Priyambodo and Srihanto, Eko Agus and Krismuniarti, Elizabeth Devi and anggraeni, Diah E. and sukatmoko, sukatmoko and handoko, mahfud (2020) A Preliminary Study on Sumatran Elephant’s Fecal DNA in Elephant Training Center, Way Kambas National Park. In: International Conference on Natural Sciences, Mathematics, Applications, Research, and Technology (ICON-SMART 2020), 22-23 October 2020., Universitas Sam Ratulangi, Manado.
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Abstract
Sumatran elephant is in the brink of extinction and its population has decreased gradually. One of the natural habitat of sumatran elephants in Lampung is in Way Kambas National Park (WKNP). The captive sumatran elephant are preserved at the WKNP’s Elephant Training Center (ETC) which face conservation challenge such as inbreeding drive [1]. The construction of a sumatran elephants molecular identity database including morphology, health status, and genetic profile needs to be done [2]. Genetic profile requires DNA in good quantity and quality. This study aimed to determine the comparison of the quality of fecal DNA of captive sumatran elephant in ETC WKNP. Grinding based dung samples were used for simple and molecular DNA extraction techniques. Dung samples of 10 individuals of sumatran elephant was carried out on the time period of 1st, 2nd, 3rd, 4th, 5th, 7th, and 14th day. The laboratory tests consisted of fecal DNA extraction followed with electrophoresis for simple technique and fecal DNA extraction, Polymerase Chain Reaction (PCR) and electrophoresis for molecular technique. The data analysis was described qualitatively.
| Item Type: | Conference or Workshop Item (Paper) |
|---|---|
| Subjects: | Q Science > Q Science (General) |
| Divisions: | Fakultas Matematika dan Ilmu Pengetahuan Alam (FMIPA) > Prodi Biologi |
| Depositing User: | Priyambodo Priyambodo |
| Date Deposited: | 18 Nov 2020 01:37 |
| Last Modified: | 18 Nov 2020 01:37 |
| URI: | http://repository.lppm.unila.ac.id/id/eprint/25950 |
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