Hapsoro, Dwi and Mayasari, Mayasari and Warganegara, Hayane Adeline and Yusnita, Yusnita (2016) Effects of Thidiazuron and Benzyladenine of Formation of Shoots and Embryogenic Nodules in Banana (Musa spp.) Tissue Culture. In: USR INTERNATIONAL SEMINAR ON FOOD SECURITY, 23 – 24 August 2016, Bandar Lampung Indonesia.
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Abstract
A Study was conducted to investigate the effect of thidiazuron (TDZ)and benzyladenine (BA) on shoot and embryogenic nodule formation in banana tissue culture. The study consisted of two experiments. The first experiment was conducted to study response of banana cv Kepok Kuning to thidiazuron (TDZ) and benzyladenine (BA) in vtro. Shoot tips were used as explants and cultured on MS medium containing MS salts, 100 mg/l myo-inositol, 0.1 mg/l thiamine-HCl, 0.5 mg/l pyridoxine-HCl, 0.5 mg/l nicotinic acid, 2 mg/l glycine, 30 g/l sucrose, combination of TDZ (0.005, 0.001, 0.05, and 0.1 mg/l) and BA (0 and 2 mg/l)as treatments. The medium was solidified with 8 g/l agar. Some cultures formed embryogenic nodules, and these structures were then cultured on MS medium supplemented with 0.1 mg/l TDZ or 0.1 mg/l TDZ + 2 mg/l BA as treatments. The second experiment aimed to investigate response of banana (cv. Raja Bulu) embryogenic nodules to TDZ and BA as previously described in the first experiment. The medium was as previously described for the first experiment. Results of the first experiment revealed that BA did not significantly affected shoot and shoot bud. The highest number of shoots was attained at 0.05 mgl TDZ (2.6 shoots per explant0. Treantment 0.1 mg/l TDZ and 0.1 mg/l TDZ + 2 mg/l BA resulted in formation of embryogenic nodules. Results of the second experiment showed that increased in TDZ concentrations did not significantly affect embryogenic nodule proliferation. In contrast, addition of 2 mg/l BA significantly decreased number of embryogenic nodules, from 12.5 to 6.2 embryogenic nodules per explant. For propagation purpose, cv. Kepok Kuning required 0.005 mg/l TDZ for shoot and shoot bud multiplication and 0.1 mgl TDZ for embryogenic nodule proliferation and cv. Raja Bulu required 0.005 mg/l TDZ for embryogenic nodule proliferation. Key Words: Benzyladenine, Kepok Kuning, Raja Bulu, shoot tips, thidiazuron.
| Item Type: | Conference or Workshop Item (Speech) |
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| Subjects: | S Agriculture > SB Plant culture |
| Divisions: | Fakultas Pertanian (FP) > Prodi Magister Agronomi |
| Depositing User: | Prof.Dr.Ir YUSNITA YUSNITA |
| Date Deposited: | 02 Jun 2017 08:22 |
| Last Modified: | 02 Jun 2017 08:22 |
| URI: | http://repository.lppm.unila.ac.id/id/eprint/2518 |
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