YULIA, MEINILWITA and Suhandy, Diding and Waluyo, Sri and sugianti, cicih (2016) DETECTION AND QUANTIFICATION OF ADULTERATION IN LUWAK COFFEE THROUGH ULTRAVIOLET-VISIBLE SPECTROSCOPY COMBINED WITH CHEMOMETRICS METHOD. In: The USR International Seminar on Food Security (UISFS), August 23 - 24, 2016, Bandar Lampung, Indonesia.

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Abstract

Luwak coffee is the most expensive coffee in the world and currently, the authentication of Luwak coffee has become very important due to the possible adulteration of Luwak coffee with non-Luwak coffee. In this research, we investigated the potential application of using ultraviolet-visible (UV-Vis) spectroscopy combined with chemometric techniques (partial least square/PLS1) for quantification of adulteration in Luwak coffee. The adulterant was Arabica coffee which was added into Luwak coffee with degree of adulteration in the range of 10-50%. A number of 30 samples were used. All samples were extracted with distilled water and filtered. The spectral acquisition was performed with 10 mm of cuvette cell using a UV - Vis spectrometer (Genesys™ 10S UV-Vis, Thermo Scientific, USA) in the range of 200-500 nm. PLS1 model correlates the actual and UV-Vis estimated values of adulterants (concentration of Arabica coffee in Luwak coffee) with coefficients of correlation (r) of 0.99 and 0.97 for calibration and validation, respectively. The low RMSECV values of 0.044 gram could be obtained. The method, therefore, is potential to be used as a rapid method for quantification of adulterant in Luwak coffee. Keywords: Luwak coffee, chemometrics, PLS1 regression, calibration, UV-Vis spectroscopy

Item Type:	Conference or Workshop Item (Paper)
Subjects:	S Agriculture > S Agriculture (General)
Divisions:	Fakultas Pertanian (FP) > Prodi Teknik Pertanian
Depositing User:	CICIH SUGI
Date Deposited:	02 Jun 2017 06:55
Last Modified:	02 Jun 2017 06:55
URI:	http://repository.lppm.unila.ac.id/id/eprint/2408

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