Imantika, Efriyan and Anggraini, Rahmi (2018) CELL SURVIVAL AND APOPTOSIS MARKER IN THE GRANULOSA CELL OF THE POLYCYSTIC OVARIAN FOLLICLE. RCOG Poster online.

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Abstract

Background Polycystic Ovarian Syndrome was characterized by chronic anovulation and or hyperandrogenism that caused by morphologic and functional disorder only in ovarian. In Indonesia there were 8 million reproductive women with polycystic ovarium. The occurance of polycystic ovarium depend on ethnic variance, obesity, insulin resistance and diabetic mellitus, thus incidence of polycystic ovarium different in many population. Infertility is the most important effect of polycystic ovarium suffered by 10-15 % couple and 40 % caused by women disorder particularly polycystic ovarium. Etiology and basic mechanism of polycystic ovarium are still unclear including several factor. Current research find that polycystic ovarium caused by disruption of apoptotic regulation and cell survival due to hyperandrogenism which is caused by hyperinsulin involving PI3-kinase Akt signalling pathway as a main pathway of insulin stimulation on its receptor. Akt fosforilate and activate target protein to stimulate growth and cell survival by activate Bad, thus Bcl-2 attach to Bad and inhibit apoptotic process. P53 regulated follicular apoptosis in ovarian follicular growth. Objective To study basic mechanism that cause apoptotic disruption of follicular atretic reduction and cell survival by compare different expression of Akt, p53, Bax and Bcl-2 protein in granulosa cell with polycystic ovarian. Method A case control study of 13 infertile patient with PCO who follow an IVF programme in Permata Hati clinic and 10 fertile patient who follow a sterilization programme in contraception clinic DR. SARDJITO hospital was performed. Granulosa cell were obtained from antral follicular fluid patient then sentrifugated to identificate protein expression by immunocitochemistry. Results Lower p53 and Bax expressions were found in granulosa cells from case group compared to control (7.4 ± 2.1 and 14.1 ± 2.2 for p53 (p=0.001)) and (6.7 ± 1.6 and 12.9 ± 2.9 for Bax (p=0.001)). Expression of Bcl-2 in case group was higher than control group (20.56 ± 4.17) whereas (13.43 ± 2.53) in control (p=0.11). Expression of Akt in case was higher than control group (16.54 ± 2.59) whereas (8.34 ± 1.00) (p=0.0007). It means that womens with lower exspression of p53 and Bax but higher expression of Akt and Bcl-2 tend to suffer infertility due to polycystic ovarium clinically. Conclusions There is significant difference in the expression of p53, Bax and Akt between infertile women with polycystic ovary and fertile women statistically. There is no significant difference in the expression of Bcl-2 statistically. Keyword: polycystic ovarium, cell survival, apoptosis

Item Type:	Article
Subjects:	R Medicine > RG Gynecology and obstetrics
Divisions:	Fakultas Kedokteran (FK) > Prodi Pendidikan Dokter
Depositing User:	EFRIYAN IM
Date Deposited:	28 May 2020 11:49
Last Modified:	28 May 2020 11:49
URI:	http://repository.lppm.unila.ac.id/id/eprint/20883

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