Harpeni, Esti and Supono, Supono and Septiani, Dwi Risca (2018) The Growth Kinetics of Bacillus Sp. D2.2 at Different pH and Salinity. Aquasains, 7 (1). pp. 647-656. ISSN 2301-816X

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Abstract

Isolate D2.2 is a bacterial isolate with 97% homology level with Bacillussp. This isolate is a biocontrol bacterium capable of inhibiting hegrowth of Gram positive bacteria and Gram negative bacteria. The use of biocontrol bacteria is one solution of disease problem in cultivation organism. Prior to being applied to the field, D2.2 isolates need to go through as eries of tests, one of which is growth kinetics testing under various nvironmental conditions, such as different degrees of pH and salinity. This is because environmental factors can affect the rapid growth of bacteria. The purpose of this study was to study thebacterial growth kinetics of D2.2 at different pH and salinity. The growth kinetics was observed by measuring optical density (OD) through a method of turbidimetry using a spectrophotometer at a wave length of 625 nm to the stage of death. Theresults showed that the fastest growth rate was found at 20 ppt salinity with 0.179 h-1 and generation time of 5,588 hours. While in all pH treatments, generation time and growth rates achieved all treatments have the same pattern. Keywords growth kinetics, Bacillus sp. D2.2, pH, salinity

Item Type:	Article
Subjects:	S Agriculture > SH Aquaculture. Fisheries. Angling
Divisions:	Fakultas Pertanian (FP) > Prodi Budidaya Perairan
Depositing User:	SUPONO
Date Deposited:	13 Apr 2020 02:06
Last Modified:	13 Apr 2020 02:06
URI:	http://repository.lppm.unila.ac.id/id/eprint/19118

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