Sukohar, Asep and HERAWATI, HENING and Witarto, Arief B. and Setiawan, Setiawan and Wirakusumah, Firman F. and Sastramihardja, Herry S. (2013) ROLE OF CHLOROGENIC ACID FROM LAMPUNG ROBUSTA COFFEE AGAINST GENE EXPRESSION OF MIRNA (MICRO RNA) 146 A ON HEPATOCELLULAR CARCINOMA CELLS. International Journal of Research in Pharmaceutical and Nano Sciences, 2 (6). pp. 776-784. ISSN 2319 – 9563

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Abstract

The use of herb to cure cancer takes a concrete breakthrough. It means that it is very essential to have a development on herb referring to a standar quality, efficacy and safety. Therefore, we need an analysis against the mechanism of herb on molecular level. Chlorogenic Acid (CA) is an active compound isolated from traditional plants from robusta coffee that is used as a chemopreventive therapy Hepatocellular Carcinoma (HCC), allegedly works as an anticancer and prevents the cell from destruction, also inhibits the growth of the cancer cells through the inhibition of free radicals. The research was done by in vitro, by using the Cell Lines Hep-G2 series 1886, obtained from Riken Cell Bank-Tohoku University. The aim of the research is how to understand the mechanism of CA in inhibiting HCC growth by using the model of Cell Lines Hep-G2 series 1886 through a series of gene expression. The type of research is experimental on 72 sample groups. 1 group consists of 250 thousands cancer cells. The research uses 3 doses of CA: 727, 500 and 250 µM, with 3 times repeated measurement, and then compared after and before the exposure. The role of those doses CA against Hep-G2 is analysed by comparing the expression of miRNA 146 A, before and after the exposure on 0, 8, 18 and 24 hours. The data is tested statistically with different test, t, repeated measurement, pearson and multiple linear regression.The lowest expression decrease of miRNA 146 A happened in the group 24 hours after the exposure at doses of 727 µ M CA (0.85), followed by 500 µM (1.28) and the highest expression increase h appened at a dose of 250 µM (1.61), continued with a statistical test at the 8th and the 18th hour Cq values miRNA 146 A significant difference p<0.05 at all doses CA (727,500 and 250 µM).The con clusion of the research is CA plays the important role on the reduction of miRNA 146 A expression.

Item Type:	Article
Subjects:	R Medicine > RM Therapeutics. Pharmacology
Divisions:	Fakultas Kedokteran (FK) > Prodi Pendidikan Dokter
Depositing User:	Dr. ASEP SUKOHAR
Date Deposited:	28 Aug 2016 13:04
Last Modified:	04 Nov 2016 03:35
URI:	http://repository.lppm.unila.ac.id/id/eprint/188

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