Andriyani, Ratih and Nukmal, Nismah and Rosa, Emantis (2016) THETOXICITY OF PURIFIED ISOLATE OF POLAR EXTRACT POWDER LEAFS GLIRICIDIA MACULATA HBR. TO CACAO MEALYBUG (PLANOCOCCUS MINOR MASKELL ). Proceeding of 3rd International Wildlife Symposium. pp. 175-181. ISSN ISBN 978-602-0860-13-8

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Abstract

One contributing factor in decreasing productivity of cocoa in the last years is due to pest attack. Cacao mealybug ( P. minor) attack the young cacao fruits, by sucking them until dry and die. Therefore it should be controlled. Alternatives pest control of the insecticide has been widely searched. G. maculata leaves consist of rich flavonoid that potencial as botanical insecticide . In oder to get the purified isolate of polar extract pouder leaf G. Maculata that named (PIGR), and test its toxicity to cacao mealybug (P. minor), the powder leaf of G. maculata were extracted by using various organic solvents (n-hexane, dichloromethane, methanol and water). A set of laboratory experiment was conducted to test the toxicity by bioassay, and to know the type and structure of PIGR by spectroscopic analysis. Five different concentrations (0 % , 0,015 % , 0,030 % , 0,045 % and 0,060 %) of PIGR with each of 3 replications were tested to cacao mealybug mortality. Mortality observed at 12 , 24 , 48 and 72 hours after treatment. Probit analysis was conducted to obtain LC 50 . The result indicated the PIGR was toxic to cacao mealybug (P. minor) with LC50 72 hours metanol extract 0,054% and water extract 0,047%. Therefore water extract more toxic than metanol extract. The toxic compound of methanol extract and water extract G. maculata is flavon with the structural frame is 2 - phenyl - 1,4 - benzopiron .

Item Type:	Article
Subjects:	Q Science > QL Zoology
Divisions:	Fakultas Matematika dan Ilmu Pengetahuan Alam (FMIPA) > Prodi Magister Ilmu Biologi
Depositing User:	Dr Emantis Rosa
Date Deposited:	29 May 2017 06:37
Last Modified:	29 May 2017 06:37
URI:	http://repository.lppm.unila.ac.id/id/eprint/1832

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