Sukmana, Irza (2013) Sandwiched polymer fibre in fibrin matrices for the dictation of endothelial cells undergoing angiogenesis. IOP science.

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Abstract

We present here a three-dimensional (3D) sandwich system made by poly(ethylene terephthalate) (PET) fibre and fibrin extracellular matrix (ECM) for endothelial cell dictation and angiogenesis guidance. In this three-dimensional system, Human Umbilical Vein Endothelial cells (HUVECs) were firstly cultured for 2 (two) days to cover the PET fibre before sandwiched in two layer fibrin gel containing HUVECs. After 4 (four) days of culture, cel-to-cel connection, tube-like structure and multi-cellular lumen formation were then assessed and validated. Phase contrast and fluorescence imaging using an inverted microscope were used to determine cell-tocell and cell-ECM interactions. Laser scanning confocal microscopy and histological techniques were used to confirm the development of tube-like structure and multi-cellular lumen formation. This study shows that polymer fibres sandwiched in fibrin gel can be used to dictate endothelial cells undergoing angiogenesis with potential application in cancer and cardiovascular study and tissue engineering vascularisation.

Item Type:	Other
Subjects:	T Technology > TS Manufactures
Divisions:	Fakultas Teknik (FT) > Prodi Teknik Mesin
Depositing User:	Dr. IRZA SUKMANA
Date Deposited:	17 Feb 2020 03:36
Last Modified:	17 Feb 2020 03:36
URI:	http://repository.lppm.unila.ac.id/id/eprint/17697

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