Ikhsanuddin, A and Rosa, Emantis and Ekowati, Christina Nugroho UJI PROTEOLITIK CENDAWAN ENTOMOPATOGEN Penicillium sp. ASAL KECOA (Periplaneta americana. In: SN-SMIAP 5 2019 FMIPA UNILA, 24 Oktober 2019, FMIPA UNILA. (Unpublished)

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Abstract

Cockroaches (Periplaneta americana )are the insect vectors of disease that can cause adverse effects on human health. Control cockroch excessive use of insecticides can lead to residus in the environment and resistance cockroach. Therefore it is necessary to control the use of alternatives such as by biologycal agents such as entomopathogenic fungal. The entomopathogenic fungi must penetrate via the integument of an cockroach to reach the hemocoel. Proteins are the molecules responsible for integument strength in cockroach, the entomopathogenic fungi must synthesis the proteases to degrading proteins. The study begins with the isolation of entomopathogenic fungi using the moist chamber method with cockroach as insect bait. Fungus that grow on cockroches are cultured and cultured and purifed on Potato Dextrose Agar (PDA ) medium and then identified. Identification was carried out through macroscopic observations including colony color and diameter and microscopic observations including conidia, conidiophores, hyphae, vesicles, fialids, and leg cells. The result of isolation and identification obtained namely is Penicillium sp. The next step from this research is proteases enzimatic activity test on PDA add anlene 1%. The clear zone formed is measured to show the activity of proteases produced by Penicillium sp.

Item Type: Conference or Workshop Item (Speech)
Subjects: Q Science > QK Botany
Divisions: Fakultas Matematika dan Ilmu Pengetahuan Alam (FMIPA) > Prodi Biologi
Depositing User: T. T. HANDAYANI
Date Deposited: 18 Nov 2019 07:20
Last Modified: 18 Nov 2019 07:20
URI: http://repository.lppm.unila.ac.id/id/eprint/16685

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