Nurcahyani, Endang and Agustrina, Rochmah and Handayani, T.T. (2016) The Protein Profile of the Plantlets of Spathoglottis plicata Bl. Induced Resistance to Fusarium oxysporum. Journal of Plant Sciences, Vol. 4 (5). pp. 102-105. ISSN 2331-0723 (Print); ISSN: 2331-0731 (Online)
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Abstract
Ground orchid (Spathoglottis plicata) cultivated have many constraints such as the appearance of fusarium wilt disease caused by Fusarium oxysporum (Fo). A resistant S. plicata plantlet to Fo has been initiated by in vitro selection on Vacin and Went (VW) medium containing fusaric acid (FA) at concentrations of 10 ppm, 20 ppm, 30 ppm, and 40 ppm, compared with controls (0 ppm), and there were indications of FA tolerant of the selected plantlets. This study aims to describe the formation mechanism of Induced Resistance by analysing the protein profile of the plantlet. The protein profile was analysed by SDS-PAGE methods. The results showed that protein profiles of explants leaves was significantly different from the control. There was an initiation of a new protein bands (± 19 kD) indicating the formation of PR-protein (peroxidase) on the plantlets which responsible for S. plicataresistant to Fo.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Protein Profile, Spathoglottis plicata Bl., In Vitro, Induced Resistance, Fusarium oxysporum |
| Subjects: | Q Science > Q Science (General) |
| Divisions: | Fakultas Matematika dan Ilmu Pengetahuan Alam (FMIPA) > Prodi Biologi |
| Depositing User: | ENDANG NURCAHYANI |
| Date Deposited: | 07 Nov 2016 06:49 |
| Last Modified: | 10 Nov 2016 02:50 |
| URI: | http://repository.lppm.unila.ac.id/id/eprint/1075 |
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