Susanto, Gregorius Nugroho and Sutyarso, Sutyarso and Rustanti, Tri (2018) Testosterone Level and Male Genital Formation of Juvenile Redclaw Crayfish After Immersion in Sea Cucumber’s Steroid Extract(SCSE). In: The International Seminar on Bioscience and Biological Education (ISBBE) 2018, 29-30 October 2018, Yogyakarta. (Submitted)

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Abstract

In the development of aquaculture, one way to increase the production rate is is through the cultivation of monosexual sex by directing genital reversal. In monosex culture, energy from reproduction is diverted to growth, resulting in larger size of spesies culture. One of the genital reversal technique is masculinization, in which the sex development is directed from female to be male. It’s only alters phenotype rather than its genotype. The redclaw crayfish, Cherax quadricarinatus is relatively new commercial commodity. Males of this species grow faster than females, so it is proposed to monosex culture of all-male population for increasing yields. The steroid hormone that commonly used in genital reversal was 17α-methyltestosterone. This synthetic hormone has a very high rate of success, but it leaves residues that are detrimental for human health, environment, and cultivated organisms. A study needs to be done to obtain a natural steroid source that is safe for humans and environment. One approach is to exploit a natural hormone extracted from the viscera of sea cucumbers (Holothuria scabra). Therefore, the purpose of the study is to determine testosterone levels in hemolymph and male formation of juvenile crayfish after immersion in the sea cucumber’s steroid extract (SCSE). The study used a Completely Random Design Factorial (CRDF) with two variables consisting of dose variations of SCSE (control, 0, 2, 4 ppm), and immersion time of 18 and 30 hours. There are 8 group treatments and each treatment with three replications. The result indicated that SCSE give an effect on increasing testosterone level and male genital formation of juvenile crayfish. There was an interaction between dose and duration time of immersion in the SCSE on testosterone levels. The highest testosterone level was found in the treatment of 4 ppm SCSE and 30 hours immersion at 0,248 ng/mL with male percentage of 77,45%. The interaction between dose and duration time of immersion SCSE had a significantly effect on the growth, biomass and testosterone levels. Keywords : testosterone, redclaw, crayfish, steroid, sea cucumber

Item Type:	Conference or Workshop Item (Speech)
Subjects:	Q Science > QP Physiology
Divisions:	Fakultas Matematika dan Ilmu Pengetahuan Alam (FMIPA) > Prodi Magister Ilmu Biologi
Depositing User:	Dr. MSc. Gregorius Nugroho Susanto
Date Deposited:	16 Nov 2018 17:32
Last Modified:	16 Nov 2018 17:32
URI:	http://repository.lppm.unila.ac.id/id/eprint/10058

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