Rustanti, Tri and Susanto, Gregorius Nugroho and Sutyarso, Sutyarso (2018) TESTOTERONE LEVELS IN HEMOLYMPH OF JUVENILE FRESHWATER CRAYFISH (Cherax quadricarinatus) AFTER IMMERSION IN SEA CUCUMBER’S STEROID EXTRACTS (Holothuria scabra J.)AT DIFFERENT DOSE AND DURATION TIME. In: The 2nd International Conference on Applied Sciences Mathematics and Informatics, 9-11 Agustus 2018, Hotel Horison, Bandar Lampung, Indonesia.

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Abstract

Red claw crayfish (Cherax quadricarinatus) is a freshwater aquaculture commodity that is currently highly demand by the community, because it has a high nutritional value. To increase the production, it can be cultivated by the method of sex reversal. This study aims 1) to determine the most effective dose and duration time of immersion in sea cucumber’s steroid extract on sex reversal of juvenile freshwater crayfish. 2) to measure testoterone levels in hemolymph of juvenile freshwater crayfish. This study used a Completely Random Design Factorial (CRDF) with two variables consisting of dose variations (0, 2, 4 ppm), and dipping time 18 and 30 hours. Treatment of 8 groups and each treatment with three replications. The result indicated that sea cucumber’s steroid extracts give an effect on the success of increasing percentage of male genital formation of juvenile freshwater crayfish and there was an interaction between dose and duration time of immersion in sea cucumber’ steroid extract on testosterone levels. The highest percentage of males was obtained at 4 ppm with 30 hours immersion time of 77,45% and the lowest was in negative control of 42,37%. The interaction between dose and duration time of immersion had a significantly effect on the growth, biomass and testosterone levels. The highest testosterone levels was found in the treatment of 4 ppm sea cucumber’s steroid extract and 30 hours immersion at 0,248 ng/mL. Keywords : freshwater crayfish, sea cucumber, extract, steroids, sex reversal

Item Type:	Conference or Workshop Item (Poster)
Subjects:	Q Science > QP Physiology
Divisions:	Fakultas Matematika dan Ilmu Pengetahuan Alam (FMIPA) > Prodi Magister Ilmu Biologi
Depositing User:	Dr. MSc. Gregorius Nugroho Susanto
Date Deposited:	16 Nov 2018 17:31
Last Modified:	16 Nov 2018 17:31
URI:	http://repository.lppm.unila.ac.id/id/eprint/10047

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