Agustian, Joni and Harun@Kamaruddin, Azlina and Aboul-Enein, Hassan (2012) Chromatographic Comparison of Atenolol Separation in Reaction Media on Cellulose tris-(3,5-dimethylphenylcarbamate) Chiral Stationary Phase Using Ultra Fast Liquid Chromatography. Chirality, 24 (5). pp. 356-367. ISSN 1520-636X
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Abstract
Because chiral liquid chromatography (LC) could become a powerful tool to estimate racemic atenolol quantity, excellent enantiomeric separation should be produced during data acquisition for satisfactory observation of atenolol concentrations throughout the racemic resolution processes. Selection of chiral LC column and analytical protocol that fulfill demands of the ultra fast LC analysis is essential. This article describes the characteristics of atenolol chromatographic separation that resulted fromdifferent resolution media and analytical protocols with the use of a ChiralcelW OD column. The chromatograms showed quite different characteristics of the separation process. The single enantiomer and racemic atenolol could be recognized by the ChiralcelW OD column in less than 20 min. Symmetrical peaks were obtained; however, several protocols produced peaks with wide bases and slanted baselines. Observations showed that efficient enantioresolution of racemic atenolol was obtained at slow mobile phase flow rate, decreased concentration of amine-type modifier but increased alcohol content in mobile phase and highest ultraviolet detection wavelength were required. The optimal ultra fast LC protocol enables to reduce and eliminate the peaks of either the atenolol solvent or the buffers and provided the highest peak intensities of both atenolol enantiomers.
| Item Type: | Article |
|---|---|
| Subjects: | T Technology > TP Chemical technology |
| Divisions: | Fakultas Teknik (FT) > Prodi Teknik Kimia |
| Depositing User: | JONI AGUST |
| Date Deposited: | 04 Oct 2016 01:12 |
| Last Modified: | 04 Oct 2016 01:12 |
| URI: | http://repository.lppm.unila.ac.id/id/eprint/711 |
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