Nurcahyani, Endang (2018) In Vivo Study: Characterization of Mutants Vanilla planifolia Andrews Resistant To Fusarium Wilt Disease Based On Analysis of the Lignin and the Phenol Content. IOSR Journal of Agriculture and Veterinary Science (IOSR-JAVS), 11 (3). pp. 15-18. ISSN e-ISSN: 2319-2380, p-ISSN: 2319-2372

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Abstract: The most production constrain on Vanilla planifolia plantation recently has been caused by foot rot disease that later influence in decreasing the yield product. This disease is caused by Fusarium oxysporum f. sp. vanillae (Fov). So far, the disease has not been successfully prohibited although some experiments had been conducted. The use of foot rot resistant cultivar has been introduced, which has high yield expected as one alternative method for controlling this disease. A resistant vanilla plantlet to Fov has been initiated by in vitro selection on medium containing fusaric acid (FA) on different concentration (90, 100, 110, and 120 ppm). In vivo, inoculation of Fov fungal isolates in vanilla mutants has been performed previously. The long-term goal in the overall study was to obtain Fov-resistant mutant seedlings. Specific targets to be achieved were characterization of V. planifolia mutant by in vivo. The stages of this research include: resistance analysis, lignin thickness and the phenol content of mutant resistant to Fusarium wilt disease by in vivo. The results showed that in vivo condition using concentration of FA of 110 ppm was effective for suppressing the growing of Fov, by intensity up to 25%, compared to the concentration of 90 ppm and 100 ppm respectively. In other words by using 110 ppm fusaric acid could increased the category criteria to resistant. There was a significant increased in the total of lignin thickness, and the phenol content, overall in line with the rising FA concentration. Keywords: Vanilla planifolia Mutant; lignin thickness; the phenol content; Fusarium oxysporum f.sp. vanillae, in vivo

Item Type: Article
Subjects: Q Science > Q Science (General)
Q Science > QK Botany
Divisions: Fakultas Matematika dan Ilmu Pengetahuan Alam (FMIPA) > Prodi Biologi
Date Deposited: 25 Apr 2018 08:29
Last Modified: 25 Apr 2018 08:29

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