Susianti, Susianti (2017) THE CYTOTOXIC EFFECT OF FOUR FRACTIONS FROM PURPLE NUTSEDGE (Cyperus rotundus L.) TUBER ESSENTIAL OIL ON THE HELA CELL. In: International Conference on Pharmaceutical and Clinical Research (ICPCR), 3 November 2017, Medan.

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ABSTRACT Objective: The aim of this research is to investigate the cytotoxic effect of four fractions from purple nutsedge tuber essential oil on HeLa cervical cancer cell line . Methods: The method used in this research was a cytotoxic test on HeLa cervical cancer cell line using an MTT assay. The cells were incubated along 24 hours with four fractions of purple nutsedge essential oil in a 96-well plate with eight series of doses (0.625-80μg/ml) for each fraction. Each dose was performed three times. After the absorbance of the cells was measured using an ELISA reader, the percentage of cell viability was calculated for each dose, followed by the calculation of the inhibitory concentration 50% rate (IC50) mean using probit regression analysis. Results: The results of this research showed that fraction 1 IC50 (mean+standard deviation) was 8.307 + 0.186 μg/ml, fraction 2 was 21.377 + 9.543 μg/ml, fraction 3 was 1,707.521+1,048.319µg/ml and fraction 4 was 4,398.836+3,476.323 µg/ml. It means that fraction 1 and 2 have strong cytotoxic effect and fraction 3 and 4 have not cytotoxic effect on HeLa cervical cancer cell line. Conclusion: The conclusions of this research are there was cytotoxic effect of fraction 1 and 2 from purple nutsedge tuber essential oil fraction on HeLa cervical cancer cell line. Keywords: Cyperus rotundus L., Purple Nutsedge, Essential Oil, Fraction, Cervical Cancer

Item Type: Conference or Workshop Item (Speech)
Subjects: R Medicine > RS Pharmacy and materia medica
Divisions: Fakultas Kedokteran (FK) > Prodi Pendidikan Dokter
Depositing User: SUSIANTI
Date Deposited: 17 Nov 2017 04:27
Last Modified: 17 Nov 2017 04:27

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