irawan, Bambang (2014) PREPARATION OF FUNGAL INOCULUM FOR LEAF LITTER COMPOSTING FROM SELECTED FUNGI. PREPARATION OF FUNGAL INOCULUM FOR LEAF LITTER COMPOSTING FROM SELECTED FUNGI, 9 (3). pp. 89-95. ISSN 1990-6145

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Abstract

Fungi are the major degraders of organic matter in natural environments, and almost every naturally occurring organic compound can be degraded by one fungus or another. An enormous range of organic compounds can be utilized by fungi, especially the major organic compounds such as cellulose, xylan and lignin. The aim of the research was to provide fungal inoculum of selected fungi as a Composting Starter Kit (CSK) to increase decomposition process of leaf litter. The fungi were isolated from decomposing leaf litter and selected based on qualitative measurement of cellulase, xylanase and ligninase activity. The result showed that there were 5 cellulolytic, 6 xylanolytic, and 2 ligninolytic isolates potentially used as inocula for leaf litter composting. Based on a halo test indicated that isolates Shj, Lht, and Lpt1 had the highest ratio for cellulase, xylanase and ligninase activity respectively. Inoculum of those three isolates were developed on sorghum seeds and were able to produce spores of Shj = 1.5 x 109, Lht = 9.3 x 107 and Lpt1 = 4.2 x 109 spores/ml. The viability test for inoculum showed the number of colonies of Shj = 9.6 x 109, Lht = 5.6 x 108 and Lpt1 = 8.2 x 106 CFUs. In conclusion, development of fungal inoculum is possibly prepared using sorghum grains inoculated with selected fungi. Keywords: leaf litter composting, inoculum, cellulase, xylanase, ligninase.

Item Type: Article
Subjects: Q Science > QK Botany
Q Science > QR Microbiology
Divisions: Fakultas Matematika dan Ilmu Pengetahuan Alam (FMIPA) > Prodi Biologi
Depositing User: BAMBANG IR
Date Deposited: 01 Nov 2017 04:29
Last Modified: 01 Nov 2017 04:29
URI: http://repository.lppm.unila.ac.id/id/eprint/4205

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