Sumardi, Sumardi and Antonius, suwanto and Maggy, Thenawidjaja and Tresnawati, Purwadaria (2005) ISOLATION AND CHARACTERIZATION OF MANNANOLYTIC THERMOPHILIC BACTERIA FROM PALM OIL SHELL AND THEIR MANNANASE ENZYME PRODUCTION PROPERTIES. Biotropia (25). pp. 1-10. ISSN 0215-6331

isolation and production mannanase (Biotropia 2005).pdf

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A mannanolytic thermophilic bacterium (L-07) was isolated from palm oil shell after 2 days of enrichment in liquid medium supplemented with 1% palm kernel meal as mannan source. Sequence analysis of 16S-rRNA indicated that L-07 was similar (98%) to Geobacillus stearothermophilus, a species of thermophilic aerobic bacteria. We found that G. stearothermophilus L-07 produced extracellular β-1,4-mannanases, but no β-manosidase and α-galactosidase activities. The growth of L-07 reached its maximum (3.0 x 106 cell/ml) at 12-20 hours, while the highest β-mannanase activity (0.52 U/ml) was observed in culture medium after 36 hours of cultivation at 60oC. The medium containing locust bean gum was the best for producing extracellular β-1,4-mannanases compared with kolang kaling, konjak, and palm kernel meal. SDS-PAGE and zymogram analysis demonstrated that crude mannanase complex of L-07 from locust bean gum containing medium comprised three active bands with molecular weight of 85, 73 and 50 kDa.

Item Type: Article
Subjects: Q Science > QR Microbiology
Divisions: Fakultas Matematika dan Ilmu Pengetahuan Alam (FMIPA) > Prodi Biologi
Depositing User: Sumardi Sumardi Sumardi
Date Deposited: 02 Jun 2020 07:59
Last Modified: 02 Jun 2020 07:59

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