Irawan, Arik and Ni Luh Gede Ratna Juliasih, Ni Luh Gede Ratna Juliasih and Hendri, John and Setiawan, Andi Bioconversion of Shrimp Shell Waste by Marine-Derived Actinomycetes to Glucosamine and Chitooligosaccharides in Solid-State Fermentation. Biodiversity. ISSN 14888386, 21600651 (Submitted)

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Abstract

Actinomycetes secrete various extracellular enzymes involved in the biodegradation of organic compounds including chitin and chitosan biopolymers. This study aims to utilize actinomycetes to convert shrimp shells into chitooligosaccharide (COS) through solid-state fermentation. In this study, three isolates of actinomycetes were selected from the deposit of the Technical Service Unit at the Integrated Laboratory of the Center for Innovation and Technology, Lampung University. Shrimp shell waste was obtained from Lempasing free market, Bandar Lampung. The morphology of actinomycetes was examined microscopically. The ability of actinomycetes to secrete chitinolytic enzymes was tested by the agar diffusion method. Cultivation of actinomycetes on shrimp shell media was carried out by the solid-state fermentation method. The results of morphological observations of the three actinomycetes isolates showed mycelia characteristics in each isolate showing the characteristics of actinomycetes. While the results of the chitinolytic test showed that strain 18D36-A1 showed a clear zone of 2 mm on 1% chitin agar colloidal media. Chromatogram interpretation of culture extract 18D36-A1 showed the formation of glucosamine and COS. Further analysis showed that the COS content tended to decrease after the fermentation process lasted for 6 days. This shows that the process of bioconversion of shrimp shells into glucosamine and COS continues to be small molecules simultaneously. Based on these data, it can be concluded that isolate 18D36-A1 was able to break down shrimp shells into glucosamine and COS. This initial information is very important for determining the design of fermentation using a continuous or batch process.

Item Type: Article
Subjects: Q Science > Q Science (General)
Depositing User: Dr. Andi Setiawan
Date Deposited: 06 Jan 2022 01:13
Last Modified: 06 Jan 2022 01:13
URI: http://repository.lppm.unila.ac.id/id/eprint/37620

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