Noviany, Noviany and Hadi, Sutopo (2021) The analysis of the secondary metabolite compounds and antioxidant activity of ethyl acetate extracts from the stems of Bouea macrophylla griff. AIP Conference Proceedings, 2370 (060015). pp. 1-6. ISSN 0094-243X

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Gandaria (Bouea macrophylla Griff) is a potential source of antioxidant compounds and this study was conducted to analyze the secondary metabolite constituents and antioxidant activity of the ethyl acetate extracted from B. macrophylla stem. The extracts were isolated and fractioned using chromatography techniques such as thin-layer chromatography (TLC) and column chromatography (CC). Moreover, the composition of the secondary metabolites was determined by Liquid Chromatography-Mass Spectrometry (LC-MS) while the antioxidant property of the extracts and fractions was obtained using the 1,1-diphenyl-2-picrylhidrazil (DPPH) method. The ethyl acetate extract was separated with CC and 8 subfractions represented by codes E1-E8 were obtained with E1 subfraction observed to have the most active fraction on the antioxidant assay at a moderate activity with an IC50 value of 232.96 ppm. E1 was further analyzed with LC-MS and two major compounds found were lauric diethanolamide and loganine with their synergy effects suggested to be the reason for the subfraction’s moderate antioxidant activity. However, E1 requires purification to determine the most active antioxidant compound isolated from ethyl acetate extract of B. macrophylla stem.

Item Type: Article
Subjects: Q Science > QD Chemistry
Divisions: Fakultas Matematika dan Ilmu Pengetahuan Alam (FMIPA) > Prodi Kimia
Depositing User: Prof. Sutopo Hadi
Date Deposited: 09 Nov 2021 01:16
Last Modified: 09 Nov 2021 01:16

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