Putri, Meishy Handerlin and Handayani, Kusuma and Abdullah, Wawan Setiawan and Arifiyanto, Achmad Screening of Extracellular Enzymes on Serratia marcescens strain MBC1. Jurnal Riset Biologi dan Aplikasinya. ISSN 2655-9927

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Abstract

Hydrolase enzymes are a class of enzymes used to break down the organic substrates into simpler molecules. Hydrolase enzymes play a role in biocontrol, industry, and public health. One of the hydrolase enzyme producers is bacteria. Serratia marcescens strain MBC1 is a collection in the Microbiology Laboratory of the Department of Biology, Faculty of Mathematics and Natural Sciences, University of Lampung which enzymatic activities haven’t been known yet. This research aimed to determine the enzymatic activity of S. marcesescens strain MBC1 that can be used as a candidate for biocontrol agents, biosurfactant producers, industry, or in the health sector. The screening was carried out using a qualitative method plate assay using selective solid media for each test, with three replications for each test. The data obtained were analyzed statistically using non-parametric Kruskall Wallis test. The lipolytic activity of the S. marcescens strain MBC1 showed high efficacy with an enzymatic index of 5.52 and amylase, cellulases, protease, mannanase, and chitinase with enzymatic indexes, respectively, 4.17, 1.50, 0.69, 0.35, and 0.27. This enzyme activity of this isolate can be used as a candidate for biological control agents, biosurfactant producers, industry, or the health sector.

Item Type:	Article
Subjects:	Q Science > QR Microbiology
Divisions:	Fakultas Matematika dan Ilmu Pengetahuan Alam (FMIPA) > Prodi Biologi
Depositing User:	Mr Achmad Arifiyanto
Date Deposited:	27 May 2021 02:29
Last Modified:	27 May 2021 02:29
URI:	http://repository.lppm.unila.ac.id/id/eprint/30728

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